A novel phytosterol isolated from Datura inoxia, RinoxiaB is a potential cure colon cancer agent by targeting BAX/Bcl2 pathway
| Autor: | Babu Gajendran, Arulvasu Chinnasamy, Krishnapriya Madhu Varier, Prabhu Durai |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Clinical Biochemistry
Pharmaceutical Science Apoptosis 01 natural sciences Biochemistry Flow cytometry Structure-Activity Relationship Western blot Cell Line Tumor Drug Discovery medicine Humans Viability assay Molecular Biology Caspase Cell Proliferation bcl-2-Associated X Protein Dose-Response Relationship Drug Molecular Structure medicine.diagnostic_test biology Plant Extracts 010405 organic chemistry Chemistry Phytosterol Cytochrome c Cell Cycle Organic Chemistry Phytosterols biology.organism_classification Antineoplastic Agents Phytogenic Molecular biology 0104 chemical sciences Datura inoxia 010404 medicinal & biomolecular chemistry Proto-Oncogene Proteins c-bcl-2 Colonic Neoplasms biology.protein Datura Molecular Medicine Drug Screening Assays Antitumor |
| Zdroj: | Bioorganic & Medicinal Chemistry. 28:115242 |
| ISSN: | 0968-0896 |
| Popis: | Plant sterols have been widely used as chemotherapeutic agents for colorectal cancer for years together. In this study, a novel phytosterol was isolated and characterized from the leaf extract of a medicinal plant, Datura inoxia and was coined as RinoxiaB (RB). This phytosterol was observed to have antiproliferative activity against human colon adenocarcinoma cells, HCT 15. The cell viability assay revealed the IC50 value of the RB as 4 µM. Moreover, RB treated cells showed prominent morphological changes dose dependently and progressively increased the number of dead cells. Additionally, results of the comet, flow cytometry, and cell cycle analysis revealed that the majority of cells were arrested in their S and G2/M phase by blocking the mitotic spindle formation. The western blot analysis (Bcl-2, BAX, Cytochrome C, Caspases 9 & 3) clearly indicated that RB has the ability to induce apoptosis by significantly upregulating (P |
| Databáze: | OpenAIRE |
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