Assessment of the bioactivity of creosote-contaminated sediment by liver biotransformation system of rainbow trout
| Autor: | Tarja Hyötyläinen, Aimo Oikari |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Geologic Sediments
Health Toxicology and Mutagenesis Biological Availability law.invention chemistry.chemical_compound law Cytochrome P-450 CYP1A1 Animals Infusions Parenteral Water pollution Salmonidae Creosote biology Public Health Environmental and Occupational Health Sediment Aquatic animal General Medicine biology.organism_classification Pollution Trout chemistry Environmental chemistry Oncorhynchus mykiss Pyrene Rainbow trout Biomarkers Water Pollutants Chemical |
| Zdroj: | Ecotoxicology and environmental safety. 44(3) |
| ISSN: | 0147-6513 |
| Popis: | A sediment site in the Lake Jamsanvesi (municipality of Petajavesi, Finland) contaminated by creosote was investigated to assess the possible ecotoxicological risks it may cause to benthic animals, including ones which may arise due to physical measures in remediating the site. It is suggested that polycyclic aromatic hydrocarbons (PAHs) are bioavailable to fish and other aquatic animals during exposure to contaminated water, sediment, and food. In order to assess toxicological risks of sediment contents to fish, juvenile rainbow trout (Onchorhynchus mykiss) were intraperitoneally dosed with extracts of the creosote-contaminated sediments and their elutriates. This was compared to pristine lake sediment spiked with creosote. Activity of CYP1A1 in trout liver was measured as ethoxyresorufin O-deethylase (EROD). When compared to vehicle controls and the pristine reference sediment (0.9-1.3 pmol/min/mg PMS protein), the extract of creosote-contaminated sediment of Lake Jamsanvesi induced EROD activity up 20-30 times with a dose of 100 mg/kg [total PAHs (mg)/(kg) in fish]. The rise of EROD was associated with increasing levels of PAH metabolites in bile, analyzed as 1-OH pyrene equivalents. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect