Virulence factors identified by Cryptococcus neoformans mutant screen differentially modulate lung immune responses and brain dissemination
| Autor: | John R. Perfect, Yafeng Qiu, Michael J. Davis, John J. Osterholzer, Michal A. Olszewski, Daniel M. Lyons, Fuyuan Wang, Xiumiao He, Anthony T. Lee, Dena L. Toffaletti, Jeremy K. Dayrit, Daniel L. Meister |
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| Rok vydání: | 2012 |
| Předmět: |
Virulence Factors
medicine.medical_treatment Genes Fungal Virulence Pathology and Forensic Medicine Fungal Proteins 03 medical and health sciences Mice 0302 clinical medicine Immune system medicine Leukocytes Macrophage Animals Humans Genetic Testing Lung 030304 developmental biology Cryptococcus neoformans 0303 health sciences Fungal protein Mice Inbred BALB C biology Lung Diseases Fungal Fungal genetics Brain Regular Article Macrophage Activation biology.organism_classification 3. Good health Disease Models Animal Cytokine medicine.anatomical_structure Immunology Mutation Cytokines Leukocyte Common Antigens Female Gene Deletion 030215 immunology |
| Zdroj: | The American journal of pathology. 181(4) |
| ISSN: | 1525-2191 |
| Popis: | Deletions of cryptococcal PIK1, RUB1, and ENA1 genes independently rendered defects in yeast survival in human CSF and within macrophages. We evaluated virulence potential of these genes by comparing wild-type Cryptococcus neoformans strain H99 with deletant and complement strains in a BALB/c mouse model of pulmonary infection. Survival of infected mice; pulmonary cryptococcal growth and pathology; immunological parameters; dissemination kinetics; and CNS pathology were examined. Deletion of each PIK1, RUB1, and ENA1 differentially reduced pulmonary growth and dissemination rates of C. neoformans and extended mice survival. Furthermore, pik1Δ induced similar pathologies to H99, however, with significantly delayed onset; rub1Δ was more efficiently contained within pulmonary macrophages and was further delayed in causing CNS dissemination/pathology; whereas ena1Δ was progressively eliminated from the lungs and did not induce pathological lesions or disseminate into the CNS. The diminished virulence of mutant strains was associated with differential modulation of pulmonary immune responses, including changes in leukocyte subsets, cytokine responses, and macrophage activation status. Compared to H99 infection, mutants induced more hallmarks of a protective Th1 immune response, rather than Th2, and more classical, rather than alternative, macrophage activation. The magnitude of immunological effects precisely corresponded to the level of virulence displayed by each strain. Thus, cryptococcal PIK1, RUB1, and ENA1 differentially contribute to cryptococcal virulence, in correlation with their differential capacity to modulate immune responses. |
| Databáze: | OpenAIRE |
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