Sugarcane Serine Peptidase Inhibitors, Serine Peptidases, and Clp Protease System Subunits Associated with Sugarcane Borer (Diatraea saccharalis) Herbivory and Wounding
| Autor: | Daniel S. Moura, Ane H Medeiros, M O Mello, Marcio C. Silva-Filho, Fabiana Bombonato Mingossi, Renata O. Dias, Renato Vicentini, Flávia P. Franco |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Diatraea saccharalis 01 natural sciences induced resistance lcsh:Chemistry Serine sugarcane Arabidopsis Plant defense against herbivory macroarray serine peptidase inhibitors serine peptidase Clp protease system plant–insect interaction lcsh:QH301-705.5 Phylogeny Spectroscopy Plant Proteins Expressed sequence tag biology Endopeptidase Clp General Medicine Saccharum Computer Science Applications Lepidoptera Biochemistry Serine Proteinase Inhibitors Protein subunit Down-Regulation Article Catalysis Host-Parasite Interactions Inorganic Chemistry 03 medical and health sciences Animals INIBIDORES DE ENZIMAS Physical and Theoretical Chemistry Molecular Biology Gene Organic Chemistry biology.organism_classification Protein Subunits 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Chaperone (protein) biology.protein 010606 plant biology & botany |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP International Journal of Molecular Sciences; Volume 17; Issue 9; Pages: 1444 International Journal of Molecular Sciences, Vol 17, Iss 9, p 1444 (2016) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms17091444 |
| Popis: | Sugarcane's (Saccharum spp.) response to Diatraea saccharalis (F.) (Lepidoptera: (Crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane Expressed Sequence Tags (ESTs) encoding serine peptidase inhibitors, serine peptidases. and Clp protease system subunits. Our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. Among the upregulated genes, nine were similar to serine peptidase inhibitors and four were similar to Bowman-Birk Inhibitors (BBIs). Phylogenetic analysis revealed that these sequences belong to a phylogenetic group of sugarcane BBIs that are potentially involved in plant defense against insect predation. The remaining four upregulated genes included serine peptidases and one homolog to the Arabidopsis AAA+ chaperone subunit ClpD, which is a member of the Clp protease system. Among the downregulated genes, five were homologous to serine peptidases and four were homologous to Arabidopsis Clp subunits (three homologous to Clp AAA+ chaperones and one to a ClpP-related ClpR subunit). Although the roles of serine peptidase inhibitors in plant defenses against herbivory have been extensively investigated, the roles of plant serine peptidases and the Clp protease system represent a new and underexplored field of study. The up- and downregulated D. saccharalis genes presented in this study may be candidate genes for the further investigation of the sugarcane response to herbivory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|