Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats
| Autor: | Xin-Guo Zhang, Guo-Di Ma, Xiao-Qian Chen, Yi-nan Peng, Xiao-Ru Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Saccharomyces cerevisiae
lcsh:TX341-641 Rhodotorula law.invention Ferrous Metal chemistry.chemical_compound cell-iron distribution law lcsh:Technology (General) Chelation Food science Sulfate iron-rich fungus biology biology.organism_classification Bioavailability screening and isolation chemistry Biochemistry visual_art visual_art.visual_art_medium identification lcsh:T1-995 Atomic absorption spectroscopy bioavailability lcsh:Nutrition. Foods and food supply Food Science Biotechnology |
| Zdroj: | Food Science and Technology, Vol 35, Iss 1, Pp 58-65 (2015) Food Science and Technology v.35 n.1 2015 Food Science and Technology (Campinas) Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA Food Science and Technology, Volume: 35, Issue: 1, Pages: 58-65, Published: MAR 2015 |
| Popis: | Iron is an essential element for nearly all living organisms, and its deficiency is the most common form of malnutrition in the world. The organic forms of trace elements are considered more bioavailable than the inorganic forms. Although Saccharomyces cerevisiae can enrich metal elements and convert inorganic iron to organic species, its tolerability and transforming capacity are limited. The aim of this study was to screen higher biomass and other iron-enriched fungi strains besides Saccharomyces cerevisiae from the natural environment. A PDA medium containing 800 μg/mL iron was used for initial screening. Fifty strains that tolerated high iron concentration were isolated from the natural environment, and only one strain, No.BY1109, grew well at Fe (II) concentration of 10,000μg/ml. According to morphological characterization, 18S rDNA sequence analysis, and biophysical and biochemical characterization, the strain No.BY1109 was identified as Rhodotorula. The iron content of No.BY1109 (10 mg Fe/g dry cell) was determined using atomic absorption spectrometry. The results of distribution of iron in the cells showed that iron ion was mainly chelated in the cell walls and vacuoles. The bioavailability in rats confirmed that strain No.BY1109 had higher absorption efficiency than that of ferrous sulfate after single dose oral administration. The present study introduces new iron supplements, and it is a basis for finding new iron supplements from natural environment. |
| Databáze: | OpenAIRE |
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