The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells
| Autor: | Yijun Huang, Junqi Ling, Xi Wei, Yuxi Zhou, Jinming Wang, Yongbiao Huo |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Abcg2
Biophysics Polymerase Chain Reaction Biochemistry Flow cytometry stomatognathic system Side population medicine Deciduous teeth ATP Binding Cassette Transporter Subfamily G Member 2 Humans Molecular Biology Cells Cultured Dental Pulp Permanent teeth medicine.diagnostic_test biology Stem Cells Cell Biology Anatomy Molecular biology Phenotype Neoplasm Proteins stomatognathic diseases medicine.anatomical_structure Deciduous biology.protein ATP-Binding Cassette Transporters Stem cell Biomarkers |
| Zdroj: | Biochemical and Biophysical Research Communications. 400:334-339 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2010.08.058 |
| Popis: | Stem cells have been identified using the DNA-binding dye Hoechst 33342 and flow cytometry (FCM) in various tissues known as the side population (SP). The present study shows, for the first time, the presence of side population cells in human deciduous dental pulp cells (DPCs). Flow cytometric identification revealed that 2% of human deciduous DPCs were SP cells and that this SP profile disappeared in the presence of verapamil. The SP marker ABCG2 protein was localized to DPCs in the cell membrane by immunofluorescence staining, and flow cytometric analysis demonstrated that 3.6% of DPCs were ABCG2-positive. Furthermore, quantitative real-time PCR proved that ABCG2 mRNA expression in DPCs isolated from human exfoliated deciduous teeth was higher than in DPCs from permanent teeth. Our findings demonstrate that DPCs from human exfoliated deciduous teeth contain a higher proportion of the SP phenotype than permanent teeth and that they may constitute a stem cell population. |
| Databáze: | OpenAIRE |
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