Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genes
| Autor: | Lei Wang, Jocelyne M. D'Souza, Peter R. Reeves |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Rhamnose Molecular Sequence Data medicine.disease_cause law.invention Microbiology chemistry.chemical_compound fluids and secretions Antigen Transferases law Escherichia Escherichia coli Genetics medicine Antigen Gene Gene Polymerase chain reaction biology O Antigens Sequence Analysis DNA General Medicine biology.organism_classification chemistry Genes Bacterial Multigene Family bacteria Bacteria |
| Zdroj: | ResearcherID |
| ISSN: | 0378-1119 |
| Popis: | Escherichia coli associated with outbreaks of gastroenteritis and hemolytic uremic syndrome include clones with O antigens O157 and O111. However, O26 has emerged as an O antigen present in pathogenic strains, particularly those implicated in cases of infantile gastroenteritis worldwide. The O26 O antigen gene cluster was sequenced. It was found to contain the genes expected for biosynthesis of nucleotide sugars l -rhamnose, N-acetyl- l -fucosamine and N-acetyl-glucosamine, as well genes for O unit flippase, O antigen polymerase and potential transferase genes. By polymerase chain reaction testing against representative strains for the 166 Escherichia coli O serogroups and some randomly selected Gram-negative bacteria, we identified three O antigen genes that are highly specific to O26. This work provides the basis for a sensitive test for the rapid detection of pathogenic clones with the O26 antigen, which has implications for public health, especially in the control of food-borne outbreaks. |
| Databáze: | OpenAIRE |
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