Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

Autor:	Oksana Aleksandrovna Zorina, Andrey Vyacheslavovich Vasilyev, Irina Alekseevna Nedorubova, Sergey Ivanovich Kutsev, Sergei N. Chvalun, E. O. Osidak, Sergey Petrovich Domogatsky, Y. D. Zagoskin, Tatyana Borisovna Bukharova, D. V. Goldshtein, Timofei E. Grigoriev, Valeriya Sergeevna Kuznetsova, Fedor Fedorovich Losev, Igor I. Babichenko, Anatoly Alekseevich Kulakov
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	collagen Polymers and Plastics Biocompatibility Composite number Organic chemistry HAP/β-TCP Bone tissue Bone morphogenetic protein 2 Article QD241-441 stomatognathic system fibronectin BMP-2 medicine Elastic modulus biology Chemistry other technology industry and agriculture General Chemistry Adhesion osteoinduction Fibronectin medicine.anatomical_structure polylactide Self-healing hydrogels biology.protein hydrogel Biomedical engineering
Zdroj:	Polymers Volume 13 Issue 22 Polymers, Vol 13, Iss 3974, p 3974 (2021)
ISSN:	2073-4360
DOI:	10.3390/polym13223974
Popis:	In dentistry, maxillofacial surgery, traumatology, and orthopedics, there is a need to use osteoplastic materials that have not only osteoinductive and osteoconductive properties but are also convenient for use. In the study, compositions based on collagen hydrogel were developed. Polylactide granules (PLA) or a traditional bone graft, a mixture of hydroxyapatite and β-tricalcium phosphate (HAP/β-TCP), were used for gel filling to improve mechanical osteoconductive properties of compositions. The mechanical tests showed that collagen hydrogels filled with 12 wt% highly porous PLA granules (elastic modulus 373 ± 55 kPa) or 35 wt% HAP/β-TCP granules (elastic modulus 451 ± 32 kPa) had optimal manipulative properties. All composite components were cytocompatible. The cell’s viability was above 90%, and the components’ structure facilitated the cell’s surface adhesion. The bone morphogenetic protein-2 (BMP-2) provided osteoinductive composition properties. It was impregnated directly into the collagen hydrogel with the addition of fibronectin or inside porous PLA granules. The implantation of a collagen hydrogel with BMP-2 and PLA granules into a critical-size calvarial defect in rats led to the formation of the most significant volume of bone tissue: 61 ± 15%. It was almost 2.5 times more than in the groups where a collagen-fibronectin hydrogel with a mixture of HAP/β-TCP (25 ± 7%) or a fibronectin-free composition with porous PLA granules impregnated with BMP-2 (23 ± 8%) were used. Subcutaneous implantation of the compositions also showed their high biocompatibility and osteogenic potential in the absence of a bone environment. Thus, the collagen-fibronectin hydrogel with BMP-2 and PLA granules has optimal biocompatibility, osteogenic, and manipulative properties.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::476b353f3dc26319d461f2f4235311d5 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.