Classification of neurons in the adult mouse cochlear nucleus: Linear discriminant analysis
| Autor: | Michael R. Kasten, Paul B. Manis, Ruili Xie |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Aging
Physiology Sensory Physiology Action Potentials Linear Discriminant Analysis Mathematical and Statistical Techniques 0302 clinical medicine Animal Cells Medicine and Health Sciences Neurons Membrane potential Principal Component Analysis 0303 health sciences Multidisciplinary Chemistry Pyramidal Cells Statistics Discriminant Analysis Afterhyperpolarization Sensory Systems Electrophysiology medicine.anatomical_structure Auditory System Physical Sciences Medicine Cellular Types Research Article Cochlear Nucleus Dorsal cochlear nucleus Ganglion Cells Science Neurophysiology Sensory system Research and Analysis Methods Membrane Potential Cochlear nucleus 03 medical and health sciences Slice preparation Neurotransmitter receptor medicine Animals Statistical Methods Cell Shape Spiral ganglion Ion channel 030304 developmental biology Auditory Pathway Biology and Life Sciences Cell Biology Neuronal Dendrites Cellular Neuroscience Multivariate Analysis Mice Inbred CBA Neuroscience Mathematics 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 10, p e0223137 (2019) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0223137 |
| Popis: | The cochlear nucleus (CN) transforms the spike trains of spiral ganglion cells into a new set of sensory representations that are essential for auditory discriminations and perception. These transformations require the coordinated activity of different classes of neurons that are embryologically derived from distinct sets of precursors. Decades of investigation have shown that the neurons of the CN are differentiated by their ion channel expression and intrinsic excitability. In the present study we have used linear discriminant analysis (LDA) to perform an unbiased analysis of measures of the responses of CN neurons to current injections to mathematically separate cells on the basis of both morphology and physiology. Recordings were made from cells in brain slices from CBA mice and a transgenic mouse line, NF107, crossed against the Ai32 line. For each cell, responses to current injections were analyzed for spike rate, spike shape (action potential height, afterhyperpolarization depth, first spike half-width), input resistance, resting membrane potential, membrane time constant, hyperpolarization-activated sag and time constant. Cells were filled with dye for morphological classification, and visually classified according to published accounts. The different morphological classes of cells were separated with the LDA. Ventral cochlear nucleus (VCN) bushy cells, planar multipolar (T-stellate) cells, and radiate multipolar (D-stellate) cells were in separate clusters, and were also separated from all of the neurons from the dorsal cochlear nucleus (DCN). Within the DCN, the pyramidal cells and tuberculoventral cells were largely separated from a distinct clusters of cartwheel cells. DCN cells fell largely within a plane in the first 3 principal axes, whereas VCN cells were in 3 clouds approximately orthogonal to this plane. VCN neurons from the two mouse strains were slightly separated, indicating either a strain dependence or the differences in slice preparation methods. We conclude that cochlear nucleus neurons can be objectively distinguished based on their intrinsic electrical properties, but that such distinctions are still best aided by morphological identification. |
| Databáze: | OpenAIRE |
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