Non-transferrin-bound iron assay system utilizing a conventional automated analyzer
| Autor: | Hiroki Tanaka, Naomi Iizuka, Motohiro Shindo, Mikihiro Fujiya, Daisuke Kato, Lynda Addo, Satoshi Ito, Mayumi Hatayama, Noriyasu Niizeki, Katsunori Sasaki, Yutaka Kohgo, Katsuya Ikuta, Yasumichi Toki, Junki Inamura, Yoshihiro Torimoto, Kotoe Shibusa |
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| Rok vydání: | 2014 |
| Předmět: |
Iron balance
Iron Clinical Biochemistry Analytical chemistry Biochemistry Chemistry Techniques Analytical Nitroso-PSAP chemistry.chemical_compound Iron toxicity Iron homeostasis Quantification Automated analyzer Iron overload Animals Humans Chromatography High Pressure Liquid Automation Laboratory chemistry.chemical_classification Chemistry Biochemistry (medical) Transferrin Nitrilotriacetic acid Diagnostic test General Medicine Organ damage Cattle Non-transferrin-bound iron |
| Zdroj: | Clinica Chimica Acta. 437:129-135 |
| ISSN: | 0009-8981 |
| Popis: | author Iron is an essential metal in the body, but its excessive accumulation causes damage in various organs through free radical production. Iron homeostasis is therefore tightly regulated. However, when iron balance collapses, such as in prolonged transfusion, transferrin (Tf) is fully saturated and non-Tf-bound iron (NTBI) appears in the serum. Monitoring serum NTBI levels is therefore crucial in assessment of the clinical status of patients with iron overload, since NTBI is associated with cellular and organ damage. Several methods for NTBI determination have been reported, but these are extremely complicated and very few laboratories can quantify NTBI at present. In this study, we established a novel assay system utilizing automated analyzers that are widely used in clinical laboratories for diagnostic testing. In this assay, NTBI is chelated by nitrilotriacetic acid (NTA), after which the iron is reduced and transferred to nitroso-PSAP, a chromogen. The assay shows excellent linearity, reproducibility, and compatibility with HPLC, one of the most reliable conventional methods for NTBI quantification. Our novel method for NTBI measurement is high-throughput and may be a useful and powerful tool in study of the physiological and clinical importance of NTBI. |
| Databáze: | OpenAIRE |
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