Polymerase-related polypeptides associated with woodchuck hepatitis core antigen (WHcAg) particles
| Autor: | Ling-Xun Duan, Marcia M. Clayton, Mark Feitelson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.drug_class
viruses Blotting Western Molecular Sequence Data Gene Products pol Hepadnaviridae Monoclonal antibody Virus law.invention Mice law Virology medicine Animals Amino Acid Sequence Antigens Viral Polymerase Mice Inbred BALB C biology Viral Core Proteins Woodchuck hepatitis virus Antibodies Monoclonal biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology digestive system diseases Blot Marmota biology.protein Recombinant DNA Female Antibody |
| Zdroj: | Virology. 180:430-433 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(91)90052-d |
| Popis: | The woodchuck hepatitis virus (WHV) polymerase (pol)-encoded polypeptide(s), obtained from purified virus nucleo-capsid particles, have been characterized by Western blotting. Peptide antibodies to amino-terminal (residues 32–45, WHV pol-6) and carboxy-terminal (residues 861–879, WHV pol-1) sequences were used, in addition to monoclonal antibodies made from purified woodchuck hepatitis core antigen (WHcAg) particles. One of the monoclonal antibodies, WC pol-11, specifically bound WHV pol-1. Both peptide and monoclonal anti-WHV pol-1 also bound a recombinant DNA-produced WHV polymerase polypeptide. These antibodies specifically detected WHcAg-associated polymerase polypeptides at 65,000 (p65) and 31,000 (p31) Da by Western blotting. These results support the conclusion that WHV pol-11 has anti-pol reactivity and that it binds the carboxyl-terminal sequences of the WHV polymerase. The finding that these reagents also specifically bind to corresponding sequences from the carboxy terminus of the hepatitis B virus polymerase suggests that these viral polymerases are cross reactive. Finally, anti-WHV pol-6 did not bind either WHcAg p65 or p31, suggesting that both of these polypeptides have different amino-terminal but the same carboxy-terminal sequences. |
| Databáze: | OpenAIRE |
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