Protective T cell immunity against respiratory syncytial virus is efficiently induced by recombinant BCG
Autor: | Kelly M. Cautivo, Eduardo Leiva, William R. Jacobs, Alexis M. Kalergis, Pablo A. González, Claudia A. Riedel, Glenn J. Fennelly, Eliseo A. Eugenin, Hugo E. Tobar, Susan M. Bueno, Jorge E. Mora |
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Rok vydání: | 2008 |
Předmět: |
Interleukin 2
viruses Respiratory Syncytial Virus Infections Biology Interferon-gamma Mice Immune system Antigen Immunity Respiratory Syncytial Virus Vaccines medicine Animals Cytotoxic T cell Antigens Viral Lung Immunity Cellular Mice Inbred BALB C Multidisciplinary Th1 Cells Viral Load Biological Sciences biochemical phenomena metabolism and nutrition respiratory system Mycobacterium bovis Virology Respiratory Syncytial Viruses Immunization Immunology Interleukin-2 bacteria Tuberculosis vaccines medicine.drug |
Zdroj: | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Artículos CONICYT CONICYT Chile instacron:CONICYT |
ISSN: | 1091-6490 0027-8424 |
Popis: | Respiratory syncytial virus (RSV) is one of the leading causes of childhood hospitalization and a major health burden worldwide. Unfortunately, because of an inefficient immunological memory, RSV infection provides limited immune protection against reinfection. Furthermore, RSV can induce an inadequate Th2-type immune response that causes severe respiratory tract inflammation and obstruction. It is thought that effective RSV clearance requires the induction of balanced Th1-type immunity, involving the activation of IFN-γ-secreting cytotoxic T cells. A recognized inducer of Th1 immunity isMycobacterium bovisbacillus Calmette–Guérin (BCG), which has been used in newborns for decades in several countries as a tuberculosis vaccine. Here, we show that immunization with recombinant BCG strains expressing RSV antigens promotes protective Th1-type immunity against RSV in mice. Activation of RSV-specific T cells producing IFN-γ and IL-2 was efficiently obtained after immunization with recombinant BCG. This type of T cell immunity was protective against RSV challenge and caused a significant reduction of inflammatory cell infiltration in the airways. Furthermore, mice immunized with recombinant BCG showed no weight loss and reduced lung viral loads. These data strongly support recombinant BCG as an efficient vaccine against RSV because of its capacity to promote protective Th1 immunity. |
Databáze: | OpenAIRE |
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