A Microfluidic Device to Establish Concentration Gradients Using Reagent Density Differences
| Autor: | Richard A. Able, Maribel Vazquez, Veronica Dudu, Qingjun Kong |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Materials science
Finite Element Analysis Microfluidics Biomedical Engineering Nanotechnology chemistry.chemical_compound Physiology (medical) Microsystem Computer Simulation Fluorescent Dyes Microelectromechanical systems Polydimethylsiloxane Pipette Dextrans Equipment Design Microfluidic Analytical Techniques Models Theoretical Device Usage Biomechanical Phenomena chemistry Reagent Hydrodynamics Microtechnology Indicators and Reagents Biological system Microfabrication |
| Zdroj: | Journal of Biomechanical Engineering. 132 |
| ISSN: | 1528-8951 0148-0731 |
| Popis: | Microfabrication has become widely utilized to generate controlled microenvironments that establish chemical concentration gradients for a variety of engineering and life science applications. To establish microfluidic flow, the majority of existing devices rely upon additional facilities, equipment, and excessive reagent supplies, which together limit device portability as well as constrain device usage to individuals trained in technological disciplines. The current work presents our laboratory-developed bridged μLane system, which is a stand-alone device that runs via conventional pipette loading and can operate for several days without need of external machinery or additional reagent volumes. The bridged μLane is a two-layer polydimethylsiloxane microfluidic device that is able to establish controlled chemical concentration gradients over time by relying solely upon differences in reagent densities. Fluorescently labeled Dextran was used to validate the design and operation of the bridged μLane by evaluating experimentally measured transport properties within the microsystem in conjunction with numerical simulations and established mathematical transport models. Results demonstrate how the bridged μLane system was used to generate spatial concentration gradients that resulted in an experimentally measured Dextran diffusivity of (0.82±0.01)×10−6 cm2/s. |
| Databáze: | OpenAIRE |
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