Circadian rhythm in intracellular Cl− activity of acutely dissociated neurons of suprachiasmatic nucleus
| Autor: | Nobutoshi Harata, Masahiko Shimura, Norio Akaike |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Nystatin medicine.medical_specialty Patch-Clamp Techniques Physiology In Vitro Techniques Biology Chlorides Internal medicine medicine Animals Circadian rhythm Rats Wistar Receptor gamma-Aminobutyric Acid Neurons Ionophores GABAA receptor Suprachiasmatic nucleus Electric Conductivity Gramicidin Intracellular Membranes Cell Biology Receptors GABA-A Circadian Rhythm Rats Electrophysiology medicine.anatomical_structure Endocrinology nervous system Hypothalamus Female Suprachiasmatic Nucleus Neuron Intracellular |
| Zdroj: | American Journal of Physiology-Cell Physiology. 282:C366-C373 |
| ISSN: | 1522-1563 0363-6143 |
| Popis: | A link between the circadian rhythm and the function of Cl−-permeable γ-aminobutyric acid (GABA) type A (GABAA) receptors on suprachiasmatic nucleus (SCN) neurons was studied by measuring intracellular activity of Cl−( aCl[Formula: see text]) at different times during a circadian cycle in SCN neurons acutely dissociated from rat brains. To measure aCl[Formula: see text], the voltage-clamp mode of the gramicidin-perforated patch-clamp technique was used, and reversal potential of GABA-induced currents ( E GABA) was converted to aCl[Formula: see text]. Measured aCl[Formula: see text] was significantly higher at around noon (20.1 ± 1.4 mM) than at three other time zones of a circadian cycle (means ranging from 11.6 to 14.3 mM). Chord conductance of GABA-induced currents showed no circadian changes, indicating a lack of circadian changes in the number or single-channel conductance of GABAA receptors. These results suggest that aCl[Formula: see text] participates in modulating GABAA receptor functions on SCN neurons during the circadian rhythm. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|