Engineered materials to model human intestinal development and cancer using organoids
| Autor: | Andrés J. García, Ricardo Cruz-Acuña |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Organogenesis Functional features Biocompatible Materials Computational biology Biology Models Biological Article 03 medical and health sciences 0302 clinical medicine Neoplasms Drug Discovery Tumor Microenvironment Organoid Animals Humans Tissue Engineering Drug discovery Extramural Neoplasms therapy Cell Differentiation Cell Biology Intestines Organoids Tissue transplantation 030104 developmental biology Drug development 030220 oncology & carcinogenesis |
| Zdroj: | Experimental Cell Research. 377:109-114 |
| ISSN: | 0014-4827 |
| Popis: | Human organoids provide constructive in vitro models of human development and disease, as these recapitulate important morphogenetic and functional features of the tissue and species of origin. However, organoid culture technologies often involve the use of biologically-derived materials (e.g. Matrigel™) that do not allow dissection of the independent contributions of the biochemical and biophysical matrix properties to organoid development. Additionally, their inherent lot-to-lot variability and, in the case of Matrigel™, tumor-derived nature limits their applicability as platforms for drug and tissue transplantation therapies. Here, we highlight recent studies that overcome these limitations through engineering of novel biomaterial platforms that (1) allow to study the independent contributions of physicochemical matrix properties to organoid development and their potential for translational therapies, and (2) better recreate the tumor microenvironment for high-throughput, pre-clinical drug development. These studies illustrate how innovative biomaterial constructs can contribute to the modeling of human development and disease using organoids, and as platforms for development of organoid-based therapies. Finally, we discuss the current limitations of the organoid field and how they can potentially be addressed using engineered biomaterials. |
| Databáze: | OpenAIRE |
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