Modulation of the venation pattern of cotyledons of transgenic tobacco for the tumorigenic 6b gene of Agrobacterium tumefaciens AKE10
| Autor: | Yasutaka Kakiuchi, Sachiko Takahashi, Hiroetsu Wabiko |
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| Rok vydání: | 2006 |
| Předmět: |
food.ingredient
Nicotiana tabacum Transgene Plant Science Lignin food Tobacco Botany medicine Gall Vein Gene Strain (chemistry) biology food and beverages Agrobacterium tumefaciens Plants Genetically Modified biology.organism_classification Cell biology medicine.anatomical_structure Genes Bacterial Seedlings cardiovascular system Cotyledon |
| Zdroj: | Journal of Plant Research. 120:259-268 |
| ISSN: | 1618-0860 0918-9440 |
| Popis: | Neoplastic plant-tissue formation, termed crown gall disease, is induced on infection with Agrobacterium tumefaciens. The tumorous tissues develop an extensive vascular system, with a venation pattern distinct from that of native host plants. We report here that the plant-tumorigenic 6b gene of the A. tumefaciens strain AKE10 is capable of inducing extensive vein formation in transgenic tobacco seedlings with distinct pattern formation. Unlike the wild-type cotyledons, transgenic cotyledons had wavy and striate veins depending on the extent of severity of leaf morphology. Graph analysis of the transgenic cotyledonous vein patterns revealed an increase in the number of branch points of veins, end-points of veins, and areas surrounded by the veins. Histological analysis showed abnormal tissue growth on the abaxial side of the cotyledon blades and continual formation of adventitious veins. These adventitiously formed veins included inverted dorso-ventrality and formation of a radial axis. |
| Databáze: | OpenAIRE |
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