Molecular and phenotypic characterization of strains nodulating Anthyllis vulneraria in mine tailings, and proposal of Aminobacter anthyllidis sp nov., the first definition of Aminobacter as legume-nodulating bacteria

Autor: Anne Willems, Souhir Soussou, Brigitte Brunel, Céline Vidal, Lionel Moulin, Jean-Claude Cleyet-Marel, Géraldine Maynaud, Lucette Mauré
Přispěvatelé: Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universiteit Gent = Ghent University [Belgium] (UGENT), ADEME, ANR-08-CESA-0002,APTITUDE,Approches pluridisciplinaires d'évaluation des performances adaptatives des bactéries du sol soumises à des stress métalliques(2008)
Rok vydání: 2012
Předmět:
[SDV]Life Sciences [q-bio]
DIVERSITY
Applied Microbiology and Biotechnology
Anthyllis vulneraria
BRADYRHIZOBIUM STRAINS
RNA
Ribosomal
16S
N-2-fixation
Phylogeny
Base Composition
0303 health sciences
Phyllobacteriaceae
Mesorhizobium
food and beverages
Fabaceae
Bacterial Typing Techniques
Housekeeping gene
Zinc
Multilocus sequence analysis
Root Nodules
Plant
Heavy-metal tolerance
DNA
Bacterial
Sequence analysis
Molecular Sequence Data
Biology
DNA
Ribosomal
Microbiology
Mining
03 medical and health sciences
Bacterial Proteins
Aminobacter
Botany
CICERI
RHIZOBIUM-LOTI
gene
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
nodA
Base Sequence
030306 microbiology
nodA gene
DNA
Sequence Analysis
DNA
16S ribosomal RNA
biology.organism_classification
SOILS
Rec A Recombinases
Lead
Acyltransferases
Zdroj: Systematic and Applied Microbiology
Systematic and Applied Microbiology, Elsevier, 2012, 35 (2), pp.65-72. ⟨10.1016/j.syapm.2011.11.002⟩
ISSN: 0723-2020
DOI: 10.1016/j.syapm.2011.11.002⟩
Popis: International audience; Bacterial strains from Zn-Pb mine tailings were isolated by trapping with Anthyllis vulneraria, a legume-host suitable for mine substratum phytostabilisation. Sequence analysis of the 16S rRNA gene and three housekeeping genes (atpD, dnaK and recA) showed that they were related to those of the genus Aminobacter. DNA-DNA relatedness of representative isolates supported the placement of novel strains in Aminobacter as a new species. Phenotypic data emphasize their differentiation from the other related species of Aminobacter and Mesorhizobium. Aminobacter isolates exhibited nodA sequences tightly related with M. loti as the closest nodA relative. By contrast, their nodA sequences were highly divergent from those of M. metallidurans, another species associated with A. vulneraria that carries two complete copies of nodA. Therefore, the novel bacterial strains efficient on A. vulneraria represented the first occurrence of legume symbionts in the genus Aminobacter. They represent a new species for which the name Aminobacter anthyllidis sp. nov. is proposed (type strain STM4645(T) = LMG26462(T) = CFBP7437(T)). (C) 2011 Elsevier GmbH. All rights reserved.
Databáze: OpenAIRE
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