Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats
| Autor: | Ebba Nexo, Eva Greibe, Christian W. Heegaard, Linda Skibsted Kornerup, Christian B. Juul, Sergey N. Fedosov |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Vitamin Male medicine.medical_specialty Medicine (miscellaneous) macromolecular substances Intestinal absorption 03 medical and health sciences chemistry.chemical_compound Kinetic modelling Transcobalamin Internal medicine hemic and lymphatic diseases Hydroxocobalamin medicine Animals Tissue Distribution Vitamin B12 Cyanocobalamin Rats Wistar Kidney 030109 nutrition & dietetics Nutrition and Dietetics fungi Skeletal muscle Original Contribution Cobalamin deficiency Rats enzymes and coenzymes (carbohydrates) Vitamin B 12 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Cattle hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | European Journal of Nutrition Kornerup, L S, Fedosov, S, Juul, C B, Greibe, E, Heegaard, C W & Nexø, E 2018, ' Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats ', European Journal of Nutrition, vol. 57, no. 4, 4, pp. 1459-1469 . https://doi.org/10.1007/s00394-017-1424-0 |
| ISSN: | 1436-6215 1436-6207 |
| DOI: | 10.1007/s00394-017-1424-0 |
| Popis: | Purpose Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[57Co]Cbl and CN[57Co]Cbl in Cbl-deficient and normal rats. Methods Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[57Co]Cbl (free or bound to bovine transcobalamin) and free CN[57Co]Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [57Co]Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods. Results Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma). Conclusions The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma. Electronic supplementary material The online version of this article (doi:10.1007/s00394-017-1424-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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