The Impact of Full-Length, Trimeric and Globular Adiponectin on Lipolysis in Subcutaneous and Visceral Adipocytes of Obese and Non-Obese Women

Autor: Michaela Tencerová, Jan Polak, Tomas Vedral, Zuzana Kovacova, Michaela Siklova-Vitkova, Vladimir Stich, Zuzana Wedellova, Lenka Rossmeislová
Rok vydání: 2013
Předmět:
Autocrine Mechanism
lcsh:Medicine
Gene Expression
Adipose tissue
AMP-Activated Protein Kinases
Cardiovascular
Biochemistry
Endocrinology
Molecular Cell Biology
Adipocytes
Protein Isoforms
Insulin
lcsh:Science
Receptor
Cells
Cultured
Multidisciplinary
Protein Kinase Signaling Cascade
Middle Aged
Signaling Cascades
Medicine
Phosphorylation
Female
Adiponectin
Receptors
Adiponectin
Research Article
Signal Transduction
Adult
Gene isoform
medicine.medical_specialty
Lipolysis
Subcutaneous Fat
Intra-Abdominal Fat
Real-Time Polymerase Chain Reaction
Internal medicine
medicine
Humans
Hypoglycemic Agents
Obesity
Protein kinase A
Biology
Nutrition
Diabetic Endocrinology
Endocrine Physiology
business.industry
lcsh:R
nutritional and metabolic diseases
AMPK
Ribonucleotides
Aminoimidazole Carboxamide
Lipid Metabolism
Metabolism
Metabolic Disorders
lcsh:Q
Protein Multimerization
business
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 6, p e66783 (2013)
ISSN: 1932-6203
Popis: Contribution of individual adiponectin isoforms to lipolysis regulation remains unknown. We investigated the impact of full-length, trimeric and globular adiponectin isoforms on spontaneous lipolysis in subcutaneous abdominal (SCAAT) and visceral adipose tissues (VAT) of obese and non-obese subjects. Furthermore, we explored the role of AMPK (5'-AMP-activated protein kinase) in adiponectin-dependent lipolysis regulation and expression of adiponectin receptors type 1 and 2 (AdipoR1 and AdipoR2) in SCAAT and VAT. Primary adipocytes isolated from SCAAT and VAT of obese and non-obese women were incubated with 20 µg/ml of: A) full-length adiponectin (physiological mixture of all adiponectin isoforms), B) trimeric adiponectin isoform or C) globular adiponectin isoform. Glycerol released into media was used as a marker of lipolysis. While full-length adiponectin inhibited lipolysis by 22% in non-obese SCAAT, globular isoform inhibited lipolysis by 27% in obese SCAAT. No effect of either isoform was detected in non-obese VAT, however trimeric isoform inhibited lipolysis by 21% in obese VAT (all p
Databáze: OpenAIRE
Externí odkaz: