Molecular characterization of Staphylococcus aureus isolates causing skin and soft tissue infections in patients from Malakand, Pakistan
| Autor: | Elke Müller, Stefan Monecke, Annett Reissig, Ralf Ehricht, S. S. Rehman, M. A. Khan, Muhammad Ali Syed, S. Madzgalla |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Staphylococcus aureus medicine.medical_specialty Penicillin binding proteins Genotype Genotyping Techniques Bacterial Toxins 030106 microbiology Exotoxins medicine.disease_cause Microbiology Tertiary Care Centers 03 medical and health sciences Medical microbiology Bacterial Proteins Leukocidins Epidemiology medicine Humans Penicillin-Binding Proteins Pakistan Genotyping Cross Infection Molecular Epidemiology Molecular epidemiology business.industry Soft Tissue Infections Nucleic Acid Hybridization General Medicine biochemical phenomena metabolism and nutrition Microarray Analysis bacterial infections and mycoses Community-Acquired Infections Infectious Diseases Staphylococcal Skin Infections business |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 35:1541-1547 |
| ISSN: | 1435-4373 0934-9723 |
| Popis: | Comparatively few studies have been published describing Staphylococcus aureus/MRSA epidemiology in Central Asia including Pakistan. Here, we report the genotyping of Staphylococcus aureus strains (that include both methicillin-susceptible and methicillin-resistant Staphylococcus aureus) from community- and hospital-acquired skin and soft-tissue infections in a tertiary care hospital in the Malakand district of the Khyber Pakhtunkhwa Province of Pakistan. Forty-five isolates of Staphylococcus aureus were characterized by microarray hybridization. Twenty isolates (44 %) were MRSA, whereas 22 (49 %) were PVL-positive. Fourteen isolates (31 %) harboured both mecA and PVL genes. The dominant clones were CC121-MSSA (n = 15, 33 %) and the PVL-positive "Bengal Bay Clone" (ST772-MRSA-V; n = 13, 29 %). The PVL-positive CC8-MRSA-IV strain "USA300" was found once. The pandemic ST239-MRSA-III strain was absent, although it has previously been observed in Pakistan. These observations require a re-assessment of schemes for initial antibiotic therapy to cover MRSA and they emphasise the need for a rapid and non-molecular test for PVL. |
| Databáze: | OpenAIRE |
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