Cord blood Lin(-)CD45(-) embryonic-like stem cells are a heterogeneous population that lack self-renewal capacity
| Autor: | Patrizia Ferretti, Sergio Querol, Alejandro Madrigal, Cesar Alvarez-Gonzalez, Susana Gómez, Richard Duggleby, Barbora Vagaska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Homeobox protein NANOG
Pluripotent Stem Cells Cytoplasm Receptors CXCR4 Hematopoietic Progenitor Cells Cellular differentiation lcsh:Medicine Cell Separation Biology Stem cell marker Regenerative Medicine SOX2 Molecular Cell Biology Humans Bone Marrow and Stem Cell Transplantation Progenitor cell Induced pluripotent stem cell lcsh:Science Cells Cultured Embryonic Stem Cells Cell Proliferation Cell Nucleus Multidisciplinary Stem Cells lcsh:R Cell Differentiation Hematology Fetal Blood Hematopoietic Stem Cells Embryonic stem cell Cell biology Hematopoiesis Immunology Leukocyte Common Antigens Medicine lcsh:Q Stem cell Cellular Types Biomarkers Research Article Developmental Biology |
| Zdroj: | PLoS ONE, Vol 8, Iss 6, p e67968 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Human umbilical cord blood (hUCB) has been proposed to contain not only haematopoietic stem cells, but also a rare pluripotent embryonic-like stem cell (ELSc) population that is negative for hematopoietic markers (Lin(-)CD45(-)) and expresses markers typical of pluripotent cells. The aim of this work was to isolate, characterise and expand this ELSc fraction from hUCB, as it may provide a valuable cell source for regenerative medicine applications. We found that we could indeed isolate a Lin(-)CD45(-) population of small cells (3-10 µm diameter) with a high nucleus to cytoplasm ratio that expressed the stem cell markers CD34 and CXCR4. However, in contrast to some previous reports, this fraction was not positive for CD133. Furthermore, although these cells expressed transcripts typical of pluripotent cells, such as SOX2, OCT3/4, and NANOG, they were not able to proliferate in any of the culture media known to support stem cell growth that we tested. Further analysis of the Lin(-)CD45(-) population by flow cytometry showed the presence of a Lin(-)CD45(-)Nestin(+) population that were also positive for CD34 (20%) but negative for CXCR4. These data suggest that the Lin(-)CD45(-) stem cell fraction present in the cord blood represents a small heterogeneous population with phenotypic characteristics of stem cells, including a Lin(-)CD45(-)Nestin(+) population not previously described. This study also suggests that heterogeneity within the Lin(-)CD45(-) cell fraction is the likely explanation for differences in the hUCB cell populations described by different groups that were isolated using different methods. These populations have been widely called "embryonic-like stem cell" on the basis of their phenotypical similarity to embryonic stem cells. However, the fact they do not seem to be able to self-renew casts some doubt on their identity, and warns against defining them as "embryonic-like stem cell" at this stage. |
| Databáze: | OpenAIRE |
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