Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in lambda phage
| Autor: | J Hiemstra, Douglas E. Berg, B R Krishnan, Suhas H. Phadnis, S Kulakauskas |
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| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial Transposable element DNA Mutational Analysis medicine.disease_cause Microbiology Genome Bacteriophage Plasmid Sequence Homology Nucleic Acid Escherichia coli medicine Insertion Cloning Molecular Molecular Biology Recombination Genetic Genetics biology Lambda phage biology.organism_classification Bacteriophage lambda Molecular biology Genes Bacterial DNA Transposable Elements Research Article Plasmids |
| Zdroj: | Journal of Bacteriology. 173:896-899 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.173.2.896-899.1991 |
| Popis: | An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants. |
| Databáze: | OpenAIRE |
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