Sequence determination of peptide by the combined use of fluoresceinisothiocyanate and phenylisothiocyanate

Autor:	Hiroshi Kawauchi, Koji Muramoto, Katura Tuzimura
Rok vydání:	1978
Předmět:	chemistry.chemical_classification chemistry.chemical_compound Chromatography chemistry Biochemistry Myoglobin Combined use Peptide General Agricultural and Biological Sciences Peptide sequence General Biochemistry Genetics and Molecular Biology Sequence determination
Zdroj:	Agricultural and Biological Chemistry. 42:1559-1563
ISSN:	1881-1280 0002-1369
DOI:	10.1271/bbb1961.42.1559
Popis:	A fluorometric manual technique has been demonstrated for determining the amino acid sequence of a polypeptide, by the combined use of fluoresceinisothiocyanate (FITC) and phenylisothiocyanate (PITC). This method permits continuous assignments of 10 residues with 5.6 nmoles of myoglobin or 23 residues with 45 nmoles.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::474a26ffcfa6f1741be95c859fffebc9 https://doi.org/10.1271/bbb1961.42.1559 Zobrazit plný text záznamu