A Competitive Inhibition Assay for Gelatin Binding Fibronectin

Autor:	Andy C. Reese, Jan E. Doran, Arlie R. Mansberger, B. Dianne Callaway, James J. Wynn
Rok vydání:	1983
Předmět:	food.ingredient biology Heparin Chemistry Blood preservation Hematology General Medicine Blood proteins Gelatin Molecular biology Fibronectins Fibronectin Microtiter plate food Non-competitive inhibition Biochemistry Blood Preservation biology.protein Humans Alkaline phosphatase Opsonin
Zdroj:	Vox Sanguinis. 45:243-251
ISSN:	1423-0410 0042-9007
DOI:	10.1111/j.1423-0410.1983.tb01910.x
Popis:	A competitive inhibition assay for functional fibronectin (Fn), based on ELISA technology, is described. The assay measures Fn's physiologic ability to bind to denatured collagen (gelatin). Affinity-purified Fn inhibits the binding of alkaline phosphatase coupled Fn to gelatin-coated wells of a microtiter plate in a concentration-dependent manner. The assay range is 50-500 micrograms Fn/ml, which is suitable for the measurement of plasma Fn in both normal and opsonin deficient individuals. It is reproducible over an eightfold dilution of plasma and is resistant to interference by normal plasma proteins. The assay described is quick, quantitative, and reproducible, and satisfies the need for a measure of functional Fn activity in the clinical laboratory.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4747ebc41d9c816b420601c1399b6b7f https://doi.org/10.1111/j.1423-0410.1983.tb01910.x Zobrazit plný text záznamu Plný text