Quantitation of plasmid DNA deposited on gold particles for particle-mediated epidermal delivery using ICP-MS
| Autor: | Bilikallahalli K. Muralidhara, Stephen Durban, Colin D. Medley, Paul Robert Mehelic, Charles W. Demarest, Steven C. Chico |
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| Rok vydání: | 2010 |
| Předmět: |
Chromatography
Analytical chemistry Reproducibility of Results DNA Biochemistry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Plasmid Drug Delivery Systems Dna genetics chemistry Plasmid dna Gold particles Nucleic acid Vaccines DNA Particle Humans Gold Epidermis Inductively coupled plasma mass spectrometry |
| Zdroj: | Analytical and bioanalytical chemistry. 398(1) |
| ISSN: | 1618-2650 |
| Popis: | DNA-plasmid-based vaccines are a promising class of next generation therapeutics. Particle-mediated epidermal delivery is an attractive method for the administration of DNA plasmid vaccines. This technology utilizes minute quantities of DNA plasmid which have been deposited onto the surface of 2-3-microm gold particles, and so the development of this technology requires the use of analytical methods that can accurately quantitate the amount of the DNA on the particle. Spectroscopic methods are generally insufficient for this task due to interference from the gold particle. ICP-MS circumvents this issue while allowing for the sensitive, reproducible, and accurate determination of the quantity of DNA on the particle surface. This report will detail the development and application of such a method. |
| Databáze: | OpenAIRE |
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