Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells

Autor: Adam D. Celiz, Jacob C. Scherba, Alaina M. Bever, Kyle H. Vining, Morgan R. Alexander, David J. Mooney
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Scaffold
Technology
Polymers
Chemistry
Multidisciplinary
02 engineering and technology
Residual monomer
ADHESION
09 Engineering
polymer microarrays
0302 clinical medicine
Dentin
multi-functional acrylates
polymer microarray
multifunctional acrylates
dental materials
General Materials Science
Cells
Cultured
02 Physical Sciences
Chemistry
Physical
Physics
SCAFFOLD
Stem Cells
Cell Differentiation
differentiation
021001 nanoscience & nanotechnology
dental pulp stem cells
Chemistry
medicine.anatomical_structure
Physics
Condensed Matter
Mechanics of Materials
Physical Sciences
Science & Technology - Other Topics
Stem cell
0210 nano-technology
03 Chemical Sciences
Materials science
Materials Science
Materials Science
Multidisciplinary
Article
Physics
Applied
03 medical and health sciences
stomatognathic system
Dental pulp stem cells
Ultimate tensile strength
medicine
Humans
Nanoscience & Nanotechnology
Dental Pulp
Cell Proliferation
Science & Technology
LESIONS
Mechanical Engineering
030206 dentistry
stomatognathic diseases
Filling materials
DISCOVERY
Pulp (tooth)
Biomedical engineering
ISSN: 0935-9648
1521-4095
Popis: Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materials that can support native functions of dental pulp stem cells (DPSCs), which are capable of regenerating dentin. A polymer microarray formed from commercially available monomers to rapidly identify materials that support DPSC adhesion is used. Based on these findings, thiol-ene chemistry is employed to achieve rapid light-curing and minimize residual monomer of the lead materials. Several triacrylate bulk polymers support DPSC adhesion, proliferation, and differentiation in vitro, and exhibit stiffness and tensile strength similar to existing dental materials. Conversely, materials composed of a trimethacrylate monomer or bisphenol A glycidyl methacrylate, which is a monomer standard in dental materials, do not support stem cell adhesion and negatively impact matrix and signaling pathways. Furthermore, thiol-ene polymerized triacrylates are used as permanent filling materials at the dentin-pulp interface in direct contact with irreversibly injured pulp tissue. These novel triacrylate-based biomaterials have potential to enable novel regenerative dental therapies in the clinic by both restoring teeth and providing a supportive niche for DPSCs.
Databáze: OpenAIRE
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