Comparison of in Vitro Cytotoxicity and Apoptogenic Activity of Magnesium Chloride and Cisplatin as Conventional Chemotherapeutic Agents in the MCF-7 Cell Line
| Autor: | Seyed Alireza Salimi-Tabatabaee, Soheila Yadollah-Damavandi, Tina Parsa, Mohammad Jafari, Seyed Abbas Mirmalek, Ehsan Jangholi, Reza Alizadeh-Navaei, Saeed Khazaei Jalil, Mohammad Amin Javidi, Hossein Ghasemzadeh Kolagar, Yekta Parsa |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cancer Research
Epidemiology Magnesium Chloride Antineoplastic Agents Apoptosis Breast Neoplasms 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences Annexin medicine Humans Cytotoxic T cell MTT assay Cytotoxicity Cell Proliferation Cisplatin Chemistry Cell growth Public Health Environmental and Occupational Health Flow Cytometry 021001 nanoscience & nanotechnology 0104 chemical sciences Oncology MCF-7 MCF-7 Cells Drug Therapy Combination Female 0210 nano-technology medicine.drug |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 17:131-134 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2016.17.s3.131 |
| Popis: | Breast cancer is the most common malignancy and also the second leading cause of cancer death among women and also in women that have a high mortality. Previous studies showed that magnesium (Mg) has cytotoxic effects on malignant cell lines. However, the anti-cancer effects of Mg on MCF-7 breast cancer cells are uncertain. This study was aimed at the comparison of the cytotoxic effect of Mg salt (MgCl2) and cisplatin on MCF-7 cells and fibroblasts (as normal cells). After treatment with various concentrations of MgCl2, and cisplatin as a positive control for 24 and 48 hours (h), cytotoxicity activity was measured by MTT assay. In addition, apoptosis was determined by annexin V/propidium iide assay. Both cisplatin and the MgCl2 exhibited dose-dependent cytotoxic effects in the MCF-7 cell line, although the LD50 of the Mg was significantly higher when compared to cispaltin (40 μg/ml vs. 20 μg/ml). Regarding annexin V/propidium results, treatment of MCF-7 cells with LD50 concentrations of cisplatin and Mg showed 59% and 44% apoptosis at 24h, respectively. Finally, the results indicated that Mg has cytotoxic effects on MCF-7 cells, but less than cisplatin as a conventional chemotherapeutic agent. However, regarding the side effects of chemotherapy drugs, it seems that Mg can be considered as a supplement for the treatment of breast cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|