Spectral karyotype analysis of colon cancer cell lines of the tumor suppressor and mutator pathway
| Autor: | H. Moerk, Wolfgang Scheppach, Hardi Luehrs, J. Koehrle, T Menzel, O. Al-Taie, Ralph Melcher, M. Scheurlen, C.R. Mueller, Michael Schmid, S. Koehler, Claus Steinlein |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
Biology medicine.disease_cause Nucleic Acid Denaturation law.invention chemistry.chemical_compound law Genetics medicine Tumor Cells Cultured Humans Genes Tumor Suppressor Molecular Biology Gene Genetics (clinical) Mutation Chromosomes Human Y Microsatellite instability Chromosome Mapping Karyotype DNA Neoplasm medicine.disease digestive system diseases Chromosome Banding chemistry Cell culture Karyotyping Colonic Neoplasms Cancer research Microsatellite Suppressor DNA |
| Zdroj: | Cytogenetic and genome research. 98(1) |
| ISSN: | 1424-8581 |
| Popis: | Background and aims: Microsatellite instability (MSI) is characterized by the size variation of microsatellites in tumor DNA as compared to matching normal DNA due to defects in the mismatch repair system. To examine the chromosomal differences in microsatellite-stable (MSS) and -unstable (MSI) tumors in detail, we analyzed MSS (Caco-2, Colo-205, SW948) and MSI (HCT-15, HCT-116, LoVo) cell lines by spectral karyotyping (SKY). Methods: SKY is a sensitive method to detect chromosome aberrations by visualizing each chromosome in a different color. Metaphases were hybridized with a SKY probe mixture. Images were visualized with the SpectraCube system and analyzed with the SKYview imaging software. Results: The average number of chromosomes was 49 in LoVo, 45 in HCT-116, 46 in HCT-15, 71 in Colo-205, 89 in Caco-2 and 66 in SW-948. Three aberrant chromosomes were detected in LoVo, three in HCT-116, two in HCT-15, seventeen in Colo-205, fourteen in Caco-2 and nine in SW948. Conclusion: The karyotypes of MSS colon cancer cells displayed complex numerical and structural aberrations. In contrast the chromosomes of MSI colon cancer cells were mostly unaltered but displayed a few isolated numerical and structural aberrations. We speculate that these isolated aberrations may be specifically involved in the pathogenesis of MSI tumors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|