PKC-δ attenuates the cancer stem cell among squamous cell carcinoma cells through down-regulating p63
| Autor: | Dongmei Zhang, Zhiqi Song, Lingyan Li, Mingjing Fu, Huan Ye, Yong-Jun Piao |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Skin Neoplasms Cell Down-Regulation Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Western blot Cancer stem cell medicine Humans Protein kinase C biology medicine.diagnostic_test Cell growth Membrane Proteins Cell Biology Transfection Molecular biology Gene Expression Regulation Neoplastic Protein Kinase C-delta stomatognathic diseases 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Carcinoma Squamous Cell Neoplastic Stem Cells biology.protein Immunohistochemistry Antibody |
| Zdroj: | Pathology - Research and Practice. 213:1119-1124 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/j.prp.2017.07.013 |
| Popis: | Protein kinase C delta (PKC-δ) has been identified as a tumor suppressor. However, the effects of PKC-δ on the cancer stem cells in squamous cell carcinomas (SCC) have not been clarified. The purpose of this study was to detect the regulation of PKC-δ on cancer stem cell among SSC cells and the role of p63 during the regulation. Immunohistochemistry of human cutaneous SCC tissues was performed to detect the expression of PKC-δ. After the human SCC13 cells infected by recombinant adenoviruses, the cell proliferation were determined. The correlation of PKC-δ and p63 was detected by western blot. The colony forming activity and the number of cancer stem cells (CSCs) in SCC identified by double-staining with anti-integrin α6 and anti-CD71 antibodies were detected. The expression of PKC-δ was obviously decreased in SCC tissues compared with that in normal skin tissues. The higher protein level of p63 in SCC was attenuated by the transfection of PKC-δ. The higher proliferation capacity of SCC13 cells, the higher activity and expression of CSCs in SCC13 cells induced by p63 were significantly suppressed by the transfection of PKC-δ. In conclusion, PKC-δ played as a protective role in SCC partly by down-regulating p63, leading to the suppression of SCC cell proliferation, attenuation of the activity and expression of CSCs in SCC cells. |
| Databáze: | OpenAIRE |
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