Development of a loop-mediated isothermal amplification (LAMP) assay for the identification of the invasive wood borer Aromia bungii (Coleoptera: Cerambycidae) from frass
| Autor: | Tommaso Bruscoli, Francesco Nugnes, Raffaele Griffo, Giovanni Cappellini, Daniele Da Lio, Linda Bartolini, Nicola Luchi, Elisabetta Rossi, Chiara Salemi, Domenico Rizzo, Antonio P. Garonna |
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| Přispěvatelé: | Rizzo, D., Luchi, N., Da Lio, D., Bartolini, L., Nugnes, F., Cappellini, G., Bruscoli, T., Salemi, C., Griffo, R. V., Garonna, A. P., Rossi, E. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Invasive pest Loop-mediated isothermal amplification Environmental Science (miscellaneous) Biology Diagnostic tools 01 natural sciences Phytosanitary survey Rapid diagnostic tool Red-necked longhorn beetle 03 medical and health sciences Prunus Red-necked longhorn beetle · Invasive pest · Rapid diagnostic tool · Phytosanitary survey Larva Aromia bungii Frass fungi Agricultural and Biological Sciences (miscellaneous) 010602 entomology Horticulture 030104 developmental biology PEST analysis Longhorn beetle Biotechnology |
| Zdroj: | 3 biotech 11 (2021). doi:10.1007/s13205-020-02602-w info:cnr-pdr/source/autori:Rizzo D., Luchi N., Da Lio D., Bartolini L., Nugnes F., Cappellini G., Bruscoli T., Salemi C., Griffo R.V., Garonna A.P., Rossi E./titolo:Development of a loop-mediated isothermal amplification (LAMP) assay for the identification of the invasive wood borer Aromia bungii (Coleoptera: Cerambycidae) from frass/doi:10.1007%2Fs13205-020-02602-w/rivista:3 biotech/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:11 |
| DOI: | 10.1007/s13205-020-02602-w |
| Popis: | The red-necked longhorn beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) is native to east Asia, where it is a major pest of cultivated and ornamental species of the genus Prunus. Morphological or molecular discrimination of adults or larval specimens is required to identify this invasive wood borer. However, recovering larval stages of the pest from trunks and branches causes extensive damage to plants and is timewasting. An alternative approach consists in applying non-invasive molecular diagnostic tools to biological traces (i.e., fecal pellets, frass). In this way, infestations in host plants can be detected without destructive methods. This paper presents a protocol based on both real-time and visual loop-mediated isothermal amplification (LAMP), using DNA of A. bungii extracted from fecal particles in larval frass. Laboratory validations demonstrated the robustness of the protocols adopted and their reliability was confirmed performing an inter-lab blind panel. The LAMP assay and the qPCR SYBR Green method using the F3/B3 LAMP external primers were equally sensitive, and both were more sensitive than the conventional PCR (sensitivity > 103 to the same starting matrix). The visual LAMP protocol, due to the relatively easy performance of the method, could be a useful tool to apply in rapid monitoring of A. bungii and in the management of its outbreaks. |
| Databáze: | OpenAIRE |
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