Isolation of Porcine Immunoglobulins and Determination of the Immunoglobulin Classes of Transmissible Gastroenteritis Viral Antibodies
| Autor: | Bohl Eh, Linda J. Saif, R. K. Paul Gupta |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Immunoglobulin A
Immunodiffusion Sucrose Swine animal diseases medicine.medical_treatment Immunology Immunoglobulins Passive immunity Biology Antibodies Viral Microbiology Chromatography DEAE-Cellulose Virus Immunoglobulin G fluids and secretions Centrifugation Density Gradient medicine Animals Immunoelectrophoresis Antiserum Gastroenteritis Transmissible of Swine Colostrum food and beverages Blood Proteins Chromatography Ion Exchange Virology Milk Infectious Diseases Immunoglobulin M Chromatography Gel biology.protein Female Parasitology Adsorption Rabbits Antibody |
| Zdroj: | Infection and Immunity. 6:600-609 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.6.4.600-609.1972 |
| Popis: | The porcine immunoglobulins M (IgM), A (IgA), and G (IgG) were isolated and purified and some of the properties of the porcine milk IgA were examined. Monospecific antisera which were prepared against these immunoglobulins in rabbits were then used to absorb a particular class of immunoglobulin from sow serum, colostrum, and milk in an attempt to identify the immunoglobulin classes of neutralizing antibodies to the porcine enteric virus, transmissible gastroenteritis (TGE). The results of these absorption studies suggest that in colostrum and milk from sows experimentally (orally) or naturally infected with live virulent TGE virus, IgA is the predominant immunoglobulin class of TGE antibodies. Both IgA and IgG TGE antibodies appeared to be present in the serum from these sows, but with IgG TGE antibodies predominating. In contrast, in the serum, colostrum and milk from sows vaccinated intramuscularly or intramammarily with live attenuated TGE virus, the TGE antibody activity was associated mainly with the IgG class of immunoglobulins. These results provide additional data indicating that the route of infection or vaccination markedly influences the immunoglobulin class of antibodies in colostrum and milk. Secondly, IgA antibodies in mammary secretions are probably essential for providing optimal passive immunity of nursing pigs against infection with TGE virus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|