Determination of urinary myo-inositol concentration by an improved enzymatic cycling method using myo-inositol dehydrogenase from Flavobacterium sp
| Autor: | Isami Tsuboi, Mamoru Takahashi, Masayuki Noritake, Shoji Kawazu, Masaru Yamakoshi, Shigeyuki Imamura, Fumio Yamagata, Takuji Kouzuma, Makoto Tominaga |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Male medicine.medical_specialty Urinary system Clinical Biochemistry Dehydrogenase Urine Biology Biochemistry Flavobacterium Sensitivity and Specificity Impaired glucose tolerance chemistry.chemical_compound Diabetes mellitus Internal medicine medicine Humans Inositol Creatinine Biochemistry (medical) General Medicine Middle Aged medicine.disease Phosphoric Monoester Hydrolases Endocrinology chemistry Female Quantitative analysis (chemistry) |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 328(1-2) |
| ISSN: | 0009-8981 |
| Popis: | Background: To determine myo-inositol more accurately, we improved the enzymatic cycling method. Methods: We screened myo-inositol dehydrogenase (MIDH; EC.1.1.1.18) from Flavobacterium sp., which was highly specific to myo-inositol. We measured urinary myo-inositol/creatinine ratio 2 h after 75-g oral glucose tolerance test (2 h MI) of 71 volunteers, and investigated the relationship between diabetes and urinary myo-inositol concentration. Results: The calibration curve was linear (r=1.00) up to 2000 μmol/l, and the detection limit was 10 μmol/l. Within-run and between-run CVs were 0.5–1.1% and 0.4–1.3%, respectively. The 2 h MI of impaired fasting glycemia (IFG; 65.1±46.6 mg/g Cr, P |
| Databáze: | OpenAIRE |
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