On-chip Cell Immobilization and Monitoring System Using Thermosensitive Gel Controlled by Suspended Polymeric Microbridge
| Autor: | Toshio Fukuda, Fumihito Arai, Yukio Magariyama, Yoko Yamanishi, Jun Teramoto, Akira Ishihama |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Microheater
Materials science Polymers Microfluidics Acrylic Resins Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Nanotechnology Saccharomyces cerevisiae law.invention chemistry.chemical_compound law Electrical and Electronic Engineering Acrylamides Microchannel Temperature technology industry and agriculture Hydrogels Equipment Design Cells Immobilized Microfluidic Analytical Techniques Lab-on-a-chip Computer Science Applications Surface micromachining chemistry Self-healing hydrogels Poly(N-isopropylacrylamide) Biotechnology Microfabrication |
| Zdroj: | IEEE Transactions on NanoBioscience. 8:312-317 |
| ISSN: | 1536-1241 |
| DOI: | 10.1109/tnb.2009.2035273 |
| Popis: | In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|