Multivalent Presentation Of Cationic Peptides On Supramolecular Nanofibers For Antimicrobial Activity
| Autor: | Ahmet Topal, Mustafa O. Guler, Aykutlu Dana, Mustafa Beter, Ayse B. Tekinay, Hatice K. Kara |
|---|---|
| Přispěvatelé: | Güler, Mustafa O. |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nanofibers Pharmaceutical Science Peptide Antimicrobial activity 01 natural sciences Epitope Epitopes Anti-Infective Agents Drug Discovery Beta sheet Multivalent presentation Priority journal chemistry.chemical_classification Antimicrobial cationic peptide Minimum inhibitory concentration Chemistry Complex formation Molecular interaction Amino acid Molecular Medicine Antibacterial activity Amphophile Bacillus subtilis Human Antibacterial peptide Supramolecular chemistry Bactericidal activity 010402 general chemistry Article Amino acid sequence 03 medical and health sciences Amphiphile Escherichia coli Peptide nanofiber Bacterial membrane Biomolecule Nanofiber Nonhuman Combinatorial chemistry 0104 chemical sciences 030104 developmental biology Glycosaminoglycan Human cell Cationic peptide Peptides Controlled study |
| Zdroj: | Molecular Pharmaceutics |
| Popis: | Noncovalent and electrostatic interactions facilitate the formation of complex networks through molecular self-assembly in biomolecules such as proteins and glycosaminoglycans. Self-assembling peptide amphiphiles (PA) are a group of molecules that can form nanofibrous structures and may contain bioactive epitopes to interact specifically with target molecules. Here, we report the presentation of cationic peptide sequences on supramolecular nanofibers formed by self-assembling peptide amphiphiles for cooperative enhanced antibacterial activity. Antibacterial properties of self-assembled peptide nanofibers were significantly higher than soluble peptide molecules with identical amino acid sequences, suggesting that the tandem presentation of bioactive epitopes is important for designing new materials for bactericidal activity. In addition, bacteria were observed to accumulate more rapidly on peptide nanofibers compared to soluble peptides, which may further enhance antibacterial activity by increasing the number of peptide molecules interacting with the bacterial membrane. The cationic peptide amphiphile nanofibers were observed to attach to bacterial membranes and disrupt their integrity. These results demonstrate that short cationic peptides show a significant improvement in antibacterial activity when presented in the nanofiber form. |
| Databáze: | OpenAIRE |
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