Study on the effect and mechanism of the dysfunction of CD4+ T cells in the disease process of chronic cardiac failure
| Autor: | Zi-Jian Ma, Xiu-Ying Lu, En-Le He, Yin-Hao Cai, Ming-Yao You |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Chronic cardiac failure Multifunction cardiogram CD4 T cells Ischemia Disease 030204 cardiovascular system & hematology Flow cytometry 03 medical and health sciences 0302 clinical medicine Text mining Statistical significance Internal medicine Medicine Medicine(all) Ejection fraction medicine.diagnostic_test business.industry General Medicine Heart function Prognosis medicine.disease 030104 developmental biology Immunology Cardiology business |
| Zdroj: | Asian Pacific Journal of Tropical Medicine. 9:682-687 |
| ISSN: | 1995-7645 |
| Popis: | Objective To study the effect and mechanism of the dysfunction of CD4+ T cells in the disease process of chronic cardiac failure (CHF). Methods According to different group technologies, 100 CHF patients were divided into the following groups: ischemia group and non-ischemia group, heart function Ⅲ–Ⅳ group and heart function Ⅰ–Ⅱ group, event group and non-event group, and 50 healthy volunteers were included in the control group. Real-time PCR was used to detect transcription factors T-bet and GATA-3 of Th1 and Th2; flow cytometry was applied to determine the ratio of Th17 and Treg cells; ELISA was employed to test cytokines IFN-γ, IL-4, IL-17 and IL-10 of peripheral blood Th1, Th2, Th17 and Treg cells, respectively; ultrasonic cardiogram was used to exploit to LVEF and LVEDd; and electrochemilu minescene immunoassay was used to examine plasma BNP. The differences of all indexes of all groups were analyzed and the correlation between CD4 T cells and clinical indexes was analyzed by Pearson correlation analysis. Results As compared to the control group, the transcription factors T-bet and GATA-3 of Th1 and Th2, the ratio of cytokines Th17 and IFN-γ, cytokines IL-17, T-bet/GATA-3, IFN-γ/IL-4, Th17 cells/Treg cells, IL-17/IL-10 of the ischemia group and non-ischemia group, heart function Ⅲ–Ⅳ group and heart function Ⅰ–Ⅱ group, event group and non-event group were all increased significantly, while their transcription factor GATA-3 of Th2, cytokines IL-4, Treg cells ratio, cytokines IL-10 were decreased obviously. The differences showed statistical significance (P |
| Databáze: | OpenAIRE |
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