Anti-aging effects of Korean Red Ginseng (KRG) in differentiated embryo chondrocyte (DEC) knockout mice
| Autor: | Seo Yule Jeong, Youn Hee Nam, Ujjal K. Bhawal, Bin Na Hong, Tong Ho Kang, Wanlapa Nuankaew, Isabel Rodriguez, Yun Hee Kim |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Senescence Aging Korean Red Ginseng Peroxisome proliferator-activated receptor Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Andrology 03 medical and health sciences 0302 clinical medicine lcsh:Botany parasitic diseases Gene expression KEGG Gene Gene knockout chemistry.chemical_classification Circadian rhythm Wild type Differentiated embryo chondrocyte lcsh:QK1-989 030104 developmental biology Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis Knockout mouse Research Article Biotechnology |
| Zdroj: | Journal of Ginseng Research, Vol 45, Iss 1, Pp 183-190 (2021) Journal of Ginseng Research |
| ISSN: | 1226-8453 |
| DOI: | 10.1016/j.jgr.2020.09.003 |
| Popis: | Background The circadian rhythm is the internal clock that controls sleep-wake cycles, metabolism, cognition, and several processes in the body, and its disruption has been associated with aging. The differentiated embryo chondrocyte (Dec) gene is related to circadian rhythm. To our knowledge, there are no reports of the relationship between dec gene expression and KRG effect. Therefore, we treated Dec gene knockout (KO) aging mice with KRG to study anti-aging related effects and possible mechanisms. Methods We evaluated KRG and expression of Dec genes in an ototoxicity model. Dec genes expression in livers of aging mice was further analyzed. Then, we assessed the effects of DEC KO on hearing function in mice by ABR. Finally, we performed DNA microarray to identify KRG-related gene expression changes in mouse liver and assessed the results using KEGG analysis. Results KRG decreased the expression of Dec genes in ototoxicity model, which may contribute to its anti-aging efficacy. Moreover, KRG suppressed Dec genes expression in liver of wild type indicating inhibition of senescence. ABR test indicated that KRG improved auditory function in aging mouse, demonstrating KRG efficacy on aging related diseases. Conclusion Finally, in KEGG analysis of 238 genes that were activated and 158 that were inhibited by KRG in DEC KO mice, activated genes were involved in proliferation signaling, mineral absorption, and PPAR signaling whereas the inhibited genes were involved in arachidonic acid metabolism and peroxisomes. Our data indicate that inhibition of senescence-related Dec genes may explain the anti-aging efficacy of KRG. |
| Databáze: | OpenAIRE |
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