Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum
| Autor: | Pushpa Dhar, Pavan Kumar, Parul Kaushal |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
Sodium arsenite Alpha-Lipoic Acid medicine.medical_treatment synaptophysin 0211 other engineering and technologies chemistry.chemical_element 02 engineering and technology Pharmacology medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine 021105 building & construction Drug Discovery medicine curcumin alpha lipoic acid lcsh:Miscellaneous systems and treatments Arsenic biology PSD95 lcsh:RZ409.7-999 030205 complementary & alternative medicine Complementary and alternative medicine chemistry Original Research Article (Experimental) Toxicity Curcumin Synaptophysin biology.protein Oxidative stress |
| Zdroj: | Journal of Ayurveda and Integrative Medicine, Vol 11, Iss 4, Pp 455-463 (2020) Journal of Ayurveda and Integrative Medicine |
| ISSN: | 0975-9476 |
| DOI: | 10.1016/j.jaim.2018.02.138 |
| Popis: | Background Arsenic is an environmental contaminant of global concern. Consumption of ground water contaminated with inorganic arsenic (iAs) continues to be the major source of its exposure. The developing nervous system is especially vulnerable to environmental insults due to its higher rate of oxygen consumption and provision of weaker antioxidant (AOX) machinery. Objective Since oxidative stress has been reported as one of the major factors underlying iAs induced toxicity, the aim of the present study is to study the effect of two AOXs i.e., Alpha Lipoic Acid (ALA) and Curcumin (Cur) in developing cerebellum of rats exposed to arsenic during postnatal period. Materials and Methods The study was carried out on mother reared neonatal rat pups grouped as normal (Ia) and sham (vehicle) controls (Ib,c,d), while the experimental groups IIa/ IIb received sodium arsenite (NaAsO2) [(1.5/2.5 mg/kg body weight (bw)] alone or along with ALA (70 mg/kg bw)- IIIa/ IIIb or along with Cur (150 mg/kg bw)- IVa/ IVb. Behavioural, biochemical and immunohistochemical procedures were carried out to understand the underlying mechanisms. Results The observations indicated deficits in locomotor function, accumulation of iAs, increased levels of oxidative stress markers along with downregulation of the expression of proteins closely associated with synaptic functioning (Synaptophysin and Postsynaptic density protein95) in the cerebellum of iAs treated animals. Substantial recovery in all these parameters was observed in AOX co-treated groups. Conclusion Our results support the potential of ALA and Cur in amelioration of iAs induced developmental neurotoxicity. ALA and Cur can be proposed as dietary adjuvants amongst populations inhabiting areas with high iAs contamination as a safe and cost effective antidotes. |
| Databáze: | OpenAIRE |
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