Plasmin-mediated fibrinolysis enables macrophage migration in a murine model of inflammation
| Autor: | Molly W. Shaw, Niki M. Moutsopoulos, Andrew Gary Lum, Eric S. Mullins, Collin Tran, Zhen Gao, Matthew J. Flick, Lakmali Munasinghage Silva, Thomas H. Bugge |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Plasmin
medicine.medical_treatment Immunology Integrin Fluorescent Antibody Technique Inflammation Biochemistry Fibrin Thrombosis and Hemostasis Immunophenotyping Leukocyte Count Mice Peritoneal cavity In vivo Fibrinolysis medicine Animals Humans Protein Interaction Domains and Motifs Fibrinolysin Mice Knockout biology Chemistry Macrophages Fibrinogen Plasminogen Chemotaxis Cell Biology Hematology Cell biology Chemotaxis Leukocyte Disease Models Animal RAW 264.7 Cells medicine.anatomical_structure biology.protein Disease Susceptibility medicine.symptom Biomarkers Protein Binding medicine.drug |
| Zdroj: | Blood |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Efficient migration of macrophages to sites of inflammation requires cell surface–bound plasmin(ogen). Here, we investigated the mechanisms underlying the deficits of plasmin(ogen)-mediated macrophage migration in 2 models: murine thioglycollate-induced peritonitis and in vitro macrophage migration. As previously reported, macrophage migration into the peritoneal cavity of mice in response to thioglycollate was significantly impaired in the absence of plasminogen. Fibrin(ogen) deposition was noted in the peritoneal cavity in response to thioglycollate, with a significant increase in fibrin(ogen) in the plasminogen-deficient mice. Interestingly, macrophage migration was restored in plasminogen-deficient mice by simultaneous imposition of fibrinogen deficiency. Consistent with this in vivo finding, chemotactic migration of cultured macrophages through a fibrin matrix did not occur in the absence of plasminogen. The macrophage requirement for plasmin-mediated fibrinolysis, both in vivo and in vitro, was negated by deletion of the major myeloid integrin αMβ2-binding motif on the γ chain of fibrin(ogen). The study identifies a critical role of fibrinolysis in macrophage migration, presumably through the alleviation of migratory constraints imposed by the interaction of leukocytes with fibrin(ogen) through the integrin αMβ2 receptor. |
| Databáze: | OpenAIRE |
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