Upregulation of microRNA-21 is a poor prognostic marker in patients with childhood B cell acute lymphoblastic leukemia
| Autor: | Ahmed A Alnagar, Hany A Labib, Neveen G. Elantouny, Nevin F Ibrahim |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Oncology medicine.medical_specialty Kaplan-Meier Estimate 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Precursor B-Cell Lymphoblastic Leukemia-Lymphoma Internal medicine microRNA Biomarkers Tumor medicine Humans Platelet Child Proportional Hazards Models Receiver operating characteristic Oncogene business.industry Age Factors Hematology B-cell acute lymphoblastic leukemia Prognosis medicine.disease Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs Treatment Outcome 030104 developmental biology Real-time polymerase chain reaction ROC Curve Case-Control Studies 030220 oncology & carcinogenesis Immunology Female business Infiltration (medical) |
| Zdroj: | Hematology. 22:392-397 |
| ISSN: | 1607-8454 |
| Popis: | Many studies have demonstrated that microRNA-21 (miR-21) is an oncogene and is upregulated in tumor tissue. However, its association with B-cell acute lymphoblastic leukemia (B-ALL) remains poorly understood.The expression of miR-21 was detected by real-time quantitative PCR in 75 children with de novo B-ALL as well as in 50 healthy controls. This study was conducted to evaluate the miR-21 as a biomarker for risk assessment, diagnosis and prognosis.Compared with normal controls, miR-21 expression was significantly upregulated in childhood B-ALL patients. Using the receiver operating characteristic curve 3.23 was selected as the cut-off value of miR-21 expression in distinguishing patients from controls. Patients group with High miR-21 expression was significantly associated with those aged2 and10 years, lower platelets count, more incidence of CNS infiltration and poorer treatment outcome also, they showed a significantly poorer disease-free survival (DFS) and overall survival (OS) compared to those with low miR-21 expression group. Its expression was an independent prognostic marker according to multivariate analysis.This is the first report demonstrating the upregulation of miR-21 in childhood B-ALL, and its association with poor response to induction therapy, shorter DFS and OS. These results suggest that miR-21 upregulation represent an unfavorable prognostic marker in Childhood B-ALL. |
| Databáze: | OpenAIRE |
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