CD147 monoclonal antibody mediated by chitosan nanoparticles loaded with α-hederin enhances antineoplastic activity and cellular uptake in liver cancer cells

Autor: Wei-liang Chen, Chun-ge Zhang, Wen-jing Zhu, Zhi-qiang Yuan, Ben-gang You, Shu-di Yang, Rong Zhu, Xue-nong Zhang, Xiao-feng Zhou, Yang Liu, Ji-zhao Li
Rok vydání: 2015
Předmět:
Intracellular Space
Apoptosis
02 engineering and technology
0302 clinical medicine
Spectroscopy
Fourier Transform Infrared
Annexin A5
Internalization
health care economics and organizations
media_common
Multidisciplinary
Microscopy
Confocal
Spectroscopy
Near-Infrared
biology
Chemistry
Liver Neoplasms
Antibodies
Monoclonal
Hep G2 Cells
021001 nanoscience & nanotechnology
Flow Cytometry
Endocytosis
Cell biology
030220 oncology & carcinogenesis
Antibody
0210 nano-technology
Intracellular
Propidium
Subcellular Fractions
Programmed cell death
medicine.drug_class
media_common.quotation_subject
education
Mice
Nude
Antineoplastic Agents
Monoclonal antibody
Fluorescence
Article
03 medical and health sciences
Imaging
Three-Dimensional
Antigen
medicine
Animals
Humans
Oleanolic Acid
Particle Size
Chitosan
technology
industry
and agriculture
Saponins
Molecular biology
Cell culture
biology.protein
Basigin
Nanoparticles
Zdroj: Scientific Reports
ISSN: 2045-2322
Popis: An antibody that specifically interacts with an antigen could be applied to an active targeting delivery system. In this study, CD147 antibody was coupled with α-hed chitosan nanoparticles (α-Hed-CS-NPs). α-Hed-CS-CD147-NPs were round and spherical in shape, with an average particle size of 148.23 ± 1.75 nm. The half-maximum inhibiting concentration (IC50) of α-Hed-CS-CD147-NPs in human liver cancer cell lines HepG2 and SMMC-7721 was lower than that of free α-Hed and α-Hed-CS-NPs. α-Hed-induced cell death was mainly triggered by apoptosis. The increase in intracellular accumulation of α-Hed-CS-CD147-NPs was also related to CD147-mediated internalization through the Caveolae-dependent pathway and lysosomal escape. The higher targeting antitumor efficacy of α-Hed-CS-CD147-NPs than that α-Hed-CS-NPs was attributed to its stronger fluorescence intensity in the tumor site in nude mice.
Databáze: OpenAIRE
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