Transcriptomic Analysis of Single Isolated Myofibers Identifies miR-27a-3p and miR-142-3p as Regulators of Metabolism in Skeletal Muscle
| Autor: | Paolo Laveder, Alessandra Zulian, Andrea Armani, Paolo Martini, Gerolamo Lanfranchi, Lisa Buson, Marco Sandri, Francesca Grespi, Francesco Chemello, Paolo Bernardi, Camilla Bean, Graziano Guella, Enrico Alessio, Etienne Hebert-Chatelain, Ruggero Ferrazza, Stefano Cagnin, Carlo Reggiani, Chiara Romualdi, Luca Scorrano, Pasqua Cancellara, Martina Bazzega |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Genetics and Molecular Biology (all) Male skeletal muscle metabolism Muscle Fibers Skeletal Mitochondrion Inbred C57BL Biochemistry Oxidative Phosphorylation Transcriptome Mice 0302 clinical medicine Myocyte Gene Regulatory Networks lcsh:QH301-705.5 Cells Cultured Cultured lipids miRNAs mitochondria single myofiber Skeletal Cell biology Mitochondria medicine.anatomical_structure Muscle Glycolysis Glycogen Cells Animals Cell Line Humans Lipid Metabolism Mice Inbred C57BL MicroRNAs Mitochondria Muscle Biology Muscle Fibers General Biochemistry Genetics and Molecular Biology 03 medical and health sciences microRNA medicine Gene Messenger RNA Biochemistry Genetics and Molecular Biology (all) Skeletal muscle Metabolism 030104 developmental biology lcsh:Biology (General) 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 26, Iss 13, Pp 3784-3797.e8 (2019) |
| ISSN: | 2211-1247 |
| Popis: | Summary: Skeletal muscle is composed of different myofiber types that preferentially use glucose or lipids for ATP production. How fuel preference is regulated in these post-mitotic cells is largely unknown, making this issue a key question in the fields of muscle and whole-body metabolism. Here, we show that microRNAs (miRNAs) play a role in defining myofiber metabolic profiles. mRNA and miRNA signatures of all myofiber types obtained at the single-cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated mouse myofiber type-specific catalog of gene and miRNA expression and establishes miR-27a-3p and miR-142-3p as regulators of lipid use in skeletal muscle. : Chemello et al. characterize coding mRNAs and non-coding microRNAs expressed by myofibers of hindlimb mouse muscles, identifying complex interactions between these molecules that modulate mitochondrial functions and muscle metabolism. They demonstrate that specific short non-coding RNAs influence the contractile fiber composition of skeletal muscles by modulating muscle metabolism. Keywords: single myofiber, skeletal muscle metabolism, mitochondria, miRNAs, lipids |
| Databáze: | OpenAIRE |
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