A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress
| Autor: | Hongbo Shao, Zhaolong Xu, Xiaolan He, Peipei Wei, Yihong Huang, Hongxiang Ma, Jinfeng Tong, Marian Brestic, Zhang Dayong, Ling Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Osmotic shock Heterologus expression Plant Science Biology lcsh:Plant culture 01 natural sciences 03 medical and health sciences chemistry.chemical_compound GmTIP2 Gene expression lcsh:SB1-1110 soybean Abscisic acid Gene Original Research Genetics Water transport promoter fungi food and beverages heterologous expression Promoter Cell biology Transmembrane domain 030104 developmental biology chemistry Heterologous expression osmotic stress 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 6 (2016) Frontiers in Plant Science |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2015.01237 |
| Popis: | Water is essential for plant growth and development. Water deficiency leads to loss of yield and decreased crop quality. To understand water transport mechanisms in plants, we cloned and characterized a novel tonoplast intrinsic protein (TIP) gene from soybean with the highest similarity to TIP2-type from other plants, and thus designated GmTIP2;3. The protein sequence contains two conserved NPA motifs and six transmembrane domains. The expression analysis indicated that this gene was constitutively expressed in all detected tissues, with higher levels in the root, stem and pod, and the accumulation of GmTIP2;3 transcript showed a significant response to osmotic stresses, including 20% PEG6000 (polyethylene glycol) and 100 μM ABA (abscisic acid) treatments. The promoter-GUS (glucuronidase) activity analysis suggested that GmTIP2;3 was also expressed in the root, stem, and leaf, and preferentially expressed in the stele of root and stem, and the core promoter region was 1000 bp in length, located upstream of the ATG start codon. The GUS tissue and induced expression observations were consistent with the findings in soybean. In addition, subcellular localization showed that GmTIP2;3 was a plasma membrane-localized protein. Yeast heterologous expression revealed that GmTIP2;3 could improve tolerance to osmotic stress in yeast cells. Integrating these results, GmTIP2;3 might play an important role in response to osmotic stress in plants. |
| Databáze: | OpenAIRE |
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