Loss of heterozygosity in the Hodgkin-Reed Sternberg cell line L1236
| Autor: | Volker Diehl, M. Kornacker, R Kurt Thomas, Thomas Zander, Jörn Bullerdiek, Nadia Massoudi, Christa Fonatsch, Juergen Wolf, Andrea Staratschek-Jox |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
medicine.medical_specialty Neoplasm Residual Tumor suppressor gene Somatic cell Loss of Heterozygosity Bone Marrow Cells Biology Allelotype Analysis Cell Line Loss of heterozygosity stomatognathic system immune system diseases hemic and lymphatic diseases medicine Tumor Cells Cultured Humans Alleles Genetics Cytogenetics Chromosome Mapping Regular Article medicine.disease tumour suppressor gene Molecular biology Hodgkin Disease Oncology Reed–Sternberg cell Cell culture Microsatellite Chromosomes Human Pair 6 Hodgkin's disease Chromosomes Human Pair 9 Chromosomes Human Pair 17 Microsatellite Repeats |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Hodgkin-Reed Sternberg cells are derived from germinal centre B-cells in most cases. Somatic mutations affecting their rearranged immunoglobulin genes were detected, rendering potential functional rearrangements non-functional. Under physiological conditions such cells would be designated to undergo apoptosis within the germinal centre. In search for the specific transforming event that prevents Hodgkin-Reed Sternberg cells from programmed cell death, cytogenetic analyses were broadly performed but did not reveal specific chromosomal aberrations. Analysis of these cells on the molecular level is difficult to perform due to the scarcity of the cells in the lymphoma tissue and the given limitations of in situ studies. To overcome these limitations, the cell line L1236, known to be derived from Hodgkin-Reed Sternberg cells in situ, was chosen for allelotype analysis. Using a panel of microsatellite loci assigned to nearly all chromosomal arms, regions of loss of heterozygosity were detected on chromosomal arms 6p, 9q and 17p. The size of lost segments was estimated by amplification of additional microsatellite loci mapped to the respective regions. Further analyses of single Hodgkin-Reed Sternberg cells will reveal whether LOH affecting these regions is a recurrent event in HD and to which extent the smallest commonly affected region can be estimated. © 2001 Cancer Research Campaign http://www.bjcancer.com |
| Databáze: | OpenAIRE |
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