Diversity structure of the microbial communities in the guts of four neotropical termite species
| Autor: | Maximiliano Ortiz, Javier Ángel Calcagno, Surendra Vikram, Maria Laura Mon, Clara Etcheverry, Don A. Cowan, Joel D. Arneodo, Paola Talia |
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| Rok vydání: | 2020 |
| Předmět: |
Firmicutes
Prokaryotae Bioinformatics Zoology lcsh:Medicine Nasutitermes corniger Isoptera Gut microbiota Gut flora Flora Microbiana Microbiology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Microbiome Termitidae 030304 developmental biology 0303 health sciences biology Ascomycota Ecology 030306 microbiology Phylum Microbial Flora General Neuroscience Microbiota lcsh:R General Medicine Biodiversity biology.organism_classification Termite species Prokaryotic and fungal diversity Microbial population biology General Agricultural and Biological Sciences Entomology Illumina amplicon sequencing |
| Zdroj: | PeerJ PeerJ, Vol 9, p e10959 (2021) PeerJ. 9 : e10959 (Abril 2021) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 2167-8359 |
| Popis: | The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic α-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut. Instituto de Biotecnología Fil: Vikram, Surendra. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Calcagno, Javier. Universidad Maimonides. Centro de Ciencias Naturales, Ambientales y Antropológicas; Argentina Fil: Ortiz, Maximiliano. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Biología de los Invertebrados; Argentina Fil: Cowan, Donald. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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