Genetic diversity ofCoccidioides posadasiifrom Brazil
| Autor: | Marcos Fábio Gadelha Rocha, Joyce Fonteles Ribeiro, Rossana de Aguiar Cordeiro, Débora de Souza Collares Maia Castelo-Branco, Rita Amanda Chaves de Lima, Zoilo Pires de Camargo, José Júlio Costa Sidrim, Thalles B. Grangeiro, Raimunda Sâmia Nogueira Brilhante |
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| Rok vydání: | 2013 |
| Předmět: |
Genotype
Molecular Sequence Data Polymorphism Single Nucleotide DNA Ribosomal Spacer parasitic diseases Genetic variation Coccidioides DNA Fungal Clade Phylogeny Genetics Genetic diversity Coccidioidomycosis Base Sequence biology Phylogenetic tree Genetic Variation Sequence Analysis DNA General Medicine biology.organism_classification DNA Fingerprinting Random Amplified Polymorphic DNA Technique Coccidioides posadasii RAPD Infectious Diseases Brazil |
| Zdroj: | Medical Mycology. 51:432-437 |
| ISSN: | 1460-2709 1369-3786 |
| DOI: | 10.3109/13693786.2012.731711 |
| Popis: | Studies of the genetic variation within populations of Coccidioides posadasii are scarce, especially for those recovered from South America. Understanding the distribution of genotypes among populations is important for epidemiological surveillance. This study evaluated the genetic diversity of 18 Brazilian strains of C. posadasii through the sequencing of the 18-28S region of nuclear rDNA, as well as through RAPD and M13-PCR fingerprinting techniques. The sequences obtained were compared to Coccidioides spp. previously deposited in GenBank. The MEGA5 program was used to perform phylogenetic analyses. Within the C. posadasii clade, a single cluster was observed, containing seven isolates from Ceará, which presented a single nucleotide polymorphism. These isolates were from the same geographical area. The strains of C. posadasii showed a lower rate of genetic diversity in the ITS1 and ITS2 regions. The results of M13 and RAPD-PCR fingerprinting indicated a similar electrophoretic profile. No differences between clinical and environmental isolates were detected. This was the first study assessing the genetic variability of a larger number of C. posadasii isolates from Brazil. |
| Databáze: | OpenAIRE |
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