Decerebrate mouse model for studies of the spinal cord circuits
| Autor: | Kyle A. Mayr, Patrick J. Whelan, Stan T. Nakanishi, C. F. Meehan, Marin Manuel |
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| Přispěvatelé: | Department of Neuroscience [Copenhagen], Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), University of Calgary, Centre de neurophysique, physiologie, pathologie (UMR 8119), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), Department of Biology, University of Hawaii at Hilo, Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cerebellum Nerve net [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Motor function General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Mice 0302 clinical medicine Medicine Animals Respiratory function Cerebrum ComputingMilieux_MISCELLANEOUS Neurons Extramural business.industry Anatomy Spinal cord 030104 developmental biology medicine.anatomical_structure Decerebration Spinal Cord Models Animal Nerve Net business Neuroscience 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Nature Protocols Nature Protocols, Nature Publishing Group, 2017, 12 (4), pp.732-747. ⟨10.1038/NPROT.2017.001⟩ Nat Protoc |
| ISSN: | 1750-2799 |
| DOI: | 10.1038/NPROT.2017.001⟩ |
| Popis: | The adult decerebrate mouse model (a mouse with the cerebrum removed) enables the study of sensory-motor integration and motor output from the spinal cord for several hours without compromising these functions with anesthesia. For example, the decerebrate mouse is ideal for examining locomotor behavior using intracellular recording approaches, which would not be possible using current anesthetized preparations. This protocol describes the steps required to achieve a low-blood-loss decerebration in the mouse and approaches for recording signals from spinal cord neurons with a focus on motoneurons. The protocol also describes an example application for the protocol: the evocation of spontaneous and actively driven stepping, including optimization of these behaviors in decerebrate mice. The time taken to prepare the animal and perform a decerebration takes ∼2 h, and the mice are viable for up to 3-8 h, which is ample time to perform most short-term procedures. These protocols can be modified for those interested in cardiovascular or respiratory function in addition to motor function and can be performed by trainees with some previous experience in animal surgery. |
| Databáze: | OpenAIRE |
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