Development and viability of bovine embryos derived from oocytes matured and fertilized in vitro and co-cultured with bovine oviducal epithelial cells
| Autor: | Keith J. Betteridge, B R Yadav, R. W. Rorie, W.A. King, L. Plante, Kangpu Xu |
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| Rok vydání: | 1992 |
| Předmět: |
Embryology
animal structures Fertilization in Vitro Andrology Embryonic and Fetal Development Endocrinology Pregnancy Animals Bovine serum albumin Fetal Viability Cells Cultured Fallopian Tubes Zygote biology Hatching Embryogenesis Obstetrics and Gynecology Embryo Embryo culture Epithelial Cells Cell Biology Embryo Transfer Embryo Mammalian Embryo transfer Blastocyst Reproductive Medicine embryonic structures Immunology biology.protein Oviduct Cattle Female |
| Zdroj: | Journal of reproduction and fertility. 94(1) |
| ISSN: | 0022-4251 |
| Popis: | A co-culture system using a suspension of detached bovine oviducal epithelial cells (BOEC) has been developed as an effective culture method for supporting the development of bovine embryos derived from oocytes matured and fertilized in vitro. Four commercially available culture media (Waymouth's, Ham's F-10, TCM 199 and Menezo's B2) supplemented with 10% oestrous cow serum, and a modified Tyrode's medium (TALP) supplemented with 0.6% bovine serum albumin were used. Menezo's B2 resulted in the highest percentages of total uncleaved presumptive zygotes, and of the cleaved zygotes that reached at least the morula stage (31-46% and 66-74%, respectively). The embryos produced in vitro in B2 with BOEC resembled embryos produced in vivo with regard to numbers of cells (averaging 45.4 in morulae, 101.5 in blastocysts, 174.7 in hatching blastocysts and 195.9 in hatched blastocysts), rate of development (hatching on Day 8-9 of culture in vitro), rate of hatching (66% of cleaved zygotes) and pregnancy rates (63%) resulting from the transcervical transfer of selected embryos. |
| Databáze: | OpenAIRE |
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