The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation
| Autor: | Vida Strasser, Janez Mravljak, Jan Přibyl, Zoran Arsov, Nadica Maltar-Strmečki, Goran Baranović, Ina Erceg, Suzana Šegota, Anja Sadžak, Manfred Kriechbaum |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology Clinical Biochemistry fluidity Biochemistry Exocytosis Article myricitrin bilayer thickness elasticity flavonols lipid peroxidation myricetin quercetin Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lipid bilayer Molecular Biology chemistry.chemical_classification Reactive oxygen species Chemistry Physics lcsh:RM1-950 Biological membrane Cell Biology 030104 developmental biology Membrane lcsh:Therapeutics. Pharmacology Biophysics Myricetin Myricitrin 030217 neurology & neurosurgery |
| Zdroj: | Antioxidants Volume 9 Issue 5 Antioxidants, Vol 9, Iss 430, p 430 (2020) |
| ISSN: | 2076-3921 |
| Popis: | The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury. |
| Databáze: | OpenAIRE |
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