Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles
| Autor: | Carla Peres de Paula, Robson Seriani, Thais Mauad, Elnara Marcia Negri, Paloma Gava Krempel, Mariangela Macchione, Marcos Antonio de Oliveira, Anderson F. Cunha, Daniela Perroni Frias |
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| Přispěvatelé: | Universidade de São Paulo (USP), Training and Development, Universidade Federal de São Carlos (UFSCar), Universidade Estadual Paulista (UNESP) |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Thioredoxin reductase Clinical Biochemistry Air pollution Bronchi Oxidative phosphorylation medicine.disease_cause Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Gene expression medicine Humans BEAS-2B Molecular Biology Cells Cultured Vehicle Emissions chemistry.chemical_classification Reactive oxygen species Epithelial Cells PRDX5 Peroxiredoxins respiratory system Diesel exhaust particles Cell biology 030104 developmental biology Gene Expression Regulation chemistry Oxidative stress 030220 oncology & carcinogenesis Particulate Matter Thioredoxin Peroxiredoxin |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0014-4800 |
| Popis: | Made available in DSpace on 2022-04-29T08:45:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Several mechanisms have been suggested to explain the adverse effects of air pollutants on airway cells. One such explanation is the presence of high concentrations of oxidants and pro-oxidants in environmental pollutants. All animal and plant cells have developed several mechanisms to prevent damage by oxidative molecules. Among these, the peroxiredoxins (PRDXs) are of interest due to a high reactivity with reactive oxygen species (ROS) through the functioning of the thioredoxin/thioredoxin reductase system. This study aimed to verify the gene expression patterns of the PRDX family in bronchial epithelial airway cells (BEAS-2B) cells exposed to diesel exhaust particles (DEPs) at a concentration of 15 μg/mL for 1 or 2 h because this it is a major component of particulate matter in the atmosphere. There was a significant decrease in mRNA fold changes of PRDX2 (0.43 ± 0.34; *p = 0.0220), PRDX5 (0.43 ± 0.34; *p = 0.0220), and PRDX6 (0.33 ± 0.25; *p = 0.0069) after 1 h of exposure to DEPs. The reduction in mRNA levels may consequently lead to a decrease in the levels of PRDX proteins, increasing oxidative stress in bronchial epithelial cells BEAS-2B and thus, negatively affecting cellular functions. Laboratory of Experimental Air Pollution (LIM05) Department of Pathology School of Medicine University of São Paulo, FMUSP- Av. Dr. Arnaldo 455, 1°floor, room 1150 PROSCED – Consulting Training and Development, Rua Alto Belo, 742, room 01 Genetic and Evolution Department Federal University of São Carlos, UFSCar- Av. Washington Luiz Km 235 Biosciences Institute Coastal Campus São Paulo State University Structural and Functional Molecular Biology, UNESP- Praça Infante Dom Henrique s/n° Laboratory for Investigations in Ophthalmology (LIM-33) University of São Paulo Medical School São Paulo, FMUSP - Dr. Arnaldo 455, 5°andar, Cerqueira César Biosciences Institute Coastal Campus São Paulo State University Structural and Functional Molecular Biology, UNESP- Praça Infante Dom Henrique s/n° FAPESP: 2011/50334-7 |
| Databáze: | OpenAIRE |
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