Investigations into Enhancing Yersinia pestis Cells Viability following Environmental Sampling for Forensic Analysis
| Autor: | Vipin K. Rastogi, Lisa S. Smith, Pooja R. Rastogi, Daniel J. Angelini, Jacquelyn Harris, Savannah Hurst, Laura Burton |
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| Rok vydání: | 2019 |
| Předmět: |
Cell Survival
Yersinia pestis 010401 analytical chemistry Forensic Sciences Temperature Biological Warfare Agents Biology biology.organism_classification 01 natural sciences Polymerase Chain Reaction 0104 chemical sciences Pathology and Forensic Medicine Microbiology Specimen Handling 03 medical and health sciences 0302 clinical medicine Genetics Environmental Microbiology Humans 030216 legal & forensic medicine Viability assay Cells Cultured |
| Zdroj: | Journal of forensic sciencesReferences. 65(4) |
| ISSN: | 1556-4029 |
| Popis: | Following an intentional or accidental bio-warfare agent (BWA) release, environmental sample analysis is absolutely critical to determine the extent of contamination. When dealing with nonspore forming BWA (e.g., Yersinia pestis), retention of cell viability is central to such analyses. Even though significant advances have been achieved in DNA sequencing technologies, a positive identification of BWAs in environmental samples must be made through the ability of cells to form colony-forming units upon culturing. Inability to revive the cells between collection and analysis renders such studies inconclusive. Commercial kits designed to preserve the viability of pathogens contained within clinical samples are available, but many of them have not been examined for their ability to preserve samples containing suspected BWAs. The study was initiated to examine the applicability of commercial solutions aiding in retention of Y. pestis viability in samples stored under nonpermissive temperatures, that is, 40 and 37°C. While none of the tested solutions sustained cell viability at 40°C, the results show five out of 17 tested preservatives were capable of supporting viability of Y. pestis at 37°C. |
| Databáze: | OpenAIRE |
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